CpGSTd3 is a lambda-cyhalothrin metabolizing glutathione S-transferase from Cydia pomonella (L.)
CpGSTd3 is a lambda-cyhalothrin metabolizing glutathione S-transferase from Cydia pomonella (L.)
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CpGSTd3 是一种来自 Cydia pomonella (L.) 的 lambda-三氯氟氰菊酯代谢谷胱甘肽 S-转移酶
DOI：
10.1021/acs.jafc.8b05432
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发表时间：
2019
影响因子：
6.1
通讯作者：
Yang Xue-Qing
中科院分区：
文献类型：
--
作者：
Wang Wei;Hu Chao;Li Xin-Ru;Wang Xiao-Qi;Yang Xue-Qing
全球检疫性水果害虫苹果小食心虫Cydia pomonella(L.)对三氟氯氰菊酯的解毒作用尚不清楚。实时荧光定量聚合酶链式反应显示，CpGSTd3在各发育阶段均有表达，幼虫期表达最丰富，卵期表达最低，四龄幼虫中肠、马氏管和成虫腹部组织中CpGSTd3的表达水平高于其他组织。用LD_(10)剂量的氯氟氰菊酯处理4龄幼虫，3h后显著诱导CpGSTd3的转录，12h后CpGSTd3的转录水平下调。在大肠杆菌中表达的重组CpGSTd3能够催化1-氯-2，4-二硝基苯(CDNB)的偶联反应，对氯氟氰菊酯的IC50值为0.65 mM。代谢实验表明，重组CpGSTd3能代谢氯氟氰菊酯。这些结果表明，CpGSTd3可能是代谢GST Inc.的氯氟氰菊酯。波蒙尼拉。
Little is known about the role of specific delta GST genes in the detoxification oflambda-cyhalothrin in the global quarantine fruit pest codling moth,Cydia pomonella(L.). Real-time quantitative PCR shows thatCpGSTd3was ubiquitously expressed at all developmental stages and is most abundant in the larval stage and lowest in the egg stage; the mRNA level ofCpGSTd3is higher in the midgut and Malpighian tubules of fourth-instar larvae and abdomens of adults than in other tissues. Exposure of fourth-instar larvae to an LD10dosage oflambda-cyhalothrin significantly induced the transcript ofCpGSTd3at 3 h, but the mRNA level was down-regulated after 12 h of treatment. Recombinant CpGSTd3 expressed inEscherichia coliwas able to catalyze the conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) and with an IC50value of 0.65 mM forlambda-cyhalothrin. Metabolism assays indicate that recombinant CpGSTd3 could metabolizelambda-cyhalothrin. These results suggest that CpGSTd3 is probably alambda-cyhalothrin metabolizing GST inC. pomonella.