Sterilization of HIV with irradiation: Relevance to infected bone allografts
Sterilization of HIV with irradiation: Relevance to infected bone allografts
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DOI：
10.1046/j.1440-1622.1999.01615.x
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发表时间：
1999-07-01
期刊：
影响因子：
--
通讯作者：
Li, P
中科院分区：
文献类型：
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作者：
Campbell, DG;Li, P
背景：同种异体骨库通常通过辐照对冷冻骨进行消毒。HN的剂量-反应关系进行计算，并确定所需的剂量，以消除可能存在于同种异体骨的病毒的生物负载determined.Methods：使用辐照冷冻HIV病毒滴度实验进行。将病毒保存在干冰（约-70 ℃）上，并暴露于钴60源，以5 kGy间隔进行0-40 kGy辐照。将淋巴细胞培养物暴露于辐射病毒的系列稀释液。结果：病毒滴度与辐射剂量呈线性关系，剂量越大，病毒滴度越高。辐照病毒灭活率为0.1134 log（10）组织培养感染剂量（50）/mL/kGy（95%可信区间0.1248-0.1020）。消除同种异体骨中HIV生物负载所需的辐照剂量为35 kGy。达到10（-6）无菌保证水平所需的辐照剂量为89 kGy。这个剂量超过了目前的建议，消毒医疗产品和目前的做法，许多bone banks.Conclusions：它的结论是，γ射线照射应被视为一个显着的病毒灭活方法骨同种异体移植。
Background: Bone allograft banks commonly sterilize frozen bone by irradiation. The dose-response relationship for HN is calculated and the dose required to inactivate the bioburden of virus that may be present in allograft bone is determined.Methods: A virus titre experiment is performed using irradiated frozen HIV. The virus is maintained on dry ice (approximately -70 degrees C) and is exposed to a cobalt 60 source with 0-40 kGy irradiation at 5 kGy intervals. Lymphocyte cell cultures are exposed to serial dilutions of the irradiated virus. The virus titre is quantified by cytological changes of HIV infection and p24 immunofluorescence.Results: There is a linear relationship between the virus titre and the radiation dose delivered. The inactivation rate of irradiated virus was 0.1134 log(10) tissue culture infective doses (50)/mL per kGy (95% confidence intervals, 0.1248-0.1020). The irradiation dose required to inactivate the HIV bioburden in allograft bone is 35 kGy. The irradiation dose required to achieve a sterility assurance level of 10(-6) is 89 kGy. This dose exceeds current recommendations for sterilizing medical products and the current practice of many bone banks.Conclusions: It is concluded that gamma irradiation should be disregarded as a significant virus inactivation method for bone allografts.