Prostaglandin E2 enhances human endometrial stromal cell differentiation.
Prostaglandin E2 enhances human endometrial stromal cell differentiation.
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DOI：
10.1210/endo.134.1.7506205
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发表时间：
1994
期刊：
影响因子：
4.8
通讯作者：
G. Frank;A. Brar;M. Cedars;S. Handwerger
中科院分区：
文献类型：
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作者：
G. Frank;A. Brar;M. Cedars;S. Handwerger
子宫内膜间质分化（蜕膜化）对于发育中的胚泡着床是必不可少的。由于前列腺素（PG）在子宫内膜中合成，并且PG结合位点已在增殖的子宫内膜基质细胞（蜕膜细胞的前体）中被证明，因此进行实验以确定PG是否参与蜕膜化过程。将人子宫内膜基质细胞在含有1 μ M醋酸甲羟孕酮（MPA）和10 nM雌二醇（含和不含PGE 2或PGF 2 α）的Dulbeco改良Eagle培养基-2%胎牛血清中培养18天。PRL的表达被用作蜕膜化的标志物。在雌二醇和MPA单独（对照）的存在下，PRL检测开始于第9天，并逐渐增加到第18天。与此相反，在PGE 2处理的细胞中在第3天检测到PRL，并且在这些细胞中在第9-12天的刺激幅度是对照细胞中的刺激幅度的1300-1400%。此外，PGE 2处理的细胞在第12天的PRL mRNA含量是对照细胞的4.6倍。PGE 2对PRL产生的影响是剂量依赖性的，最小有效剂量为10（-10）M。在没有类固醇的情况下，PGE 2对PRL产生的影响最小。与PGE 2相反，PGF 2 α处理对类固醇处理的细胞中的PRL表达没有影响。这些结果表明，PGE 2，雌二醇和MPA之间存在协同作用，导致子宫内膜间质细胞分化加速和PRL表达增强。
Endometrial stromal differentiation (decidualization) is essential for implantation of the developing blastocyst. Because prostaglandins (PGs) are synthesized in the endometrium, and PG-binding sites have been demonstrated in the proliferative endometrial stromal cell (the precursor of the decidual cell), experiments were performed to determine whether PGs are involved in the process of decidualization. Human endometrial stromal cells were cultured for 18 days in Dulbeco's Modified Eagle's Medium-2% fetal bovine serum with 1 microM medroxyprogesterone acetate (MPA) and 10 nM estradiol, with and without PGE2 or PGF2 alpha. Expression of PRL was used as a marker of decidualization. In the presence of estradiol and MPA alone (control), PRL was detected beginning on day 9 and gradually increased through day 18. In contrast, PRL was detected on day 3 in the PGE2-treated cells, and the magnitude of stimulation in these cells on days 9-12 was 1300-1400% of that in control cells. Furthermore, the PRL mRNA content of the PGE2-treated cells on day 12 was 4.6-fold greater than that in the control cells. The effect of PGE2 on PRL production was dose dependent, with a minimal effective dose of 10(-10) M. PGE2 in the absence of steroids had a minimal effect on PRL production. In contrast to PGE2, PGF2 alpha treatment had no effect on PRL expression in steroid-treated cells. These results indicate that there are synergistic effects among PGE2, estradiol, and MPA, resulting in acceleration of endometrial stromal cell differentiation and enhanced PRL expression.