Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation.
Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation.
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DOI：
10.1002/0471142735.im0409s84
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发表时间：
2009-02-01
影响因子：
--
通讯作者：
Warren, Hilary S
中科院分区：
文献类型：
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作者：
Parish, Christopher R;Glidden, Megan H;Warren, Hilary S
细胞内荧光染料5-（和-6）-羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）稳定掺入细胞中提供了监测细胞迁移和定量细胞分裂的有力工具，因为子细胞中荧光标记的顺序减少。CFSE标记的淋巴细胞已被用于分析细胞分裂和细胞功能分化之间的关系，以及细胞增殖与凋亡，在体内和体外，并允许在体内对抗原的反应位点的分析。
The stable incorporation of the intracellular fluorescent dye 5-(and -6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) into cells provides a powerful tool to monitor cell migration, and to quantify cell division, because of the sequential decrease in fluorescent labeling in daughter cells. CFSE-labeled lymphocytes have been used to analyze the relationship between cell division and differentiation of cell function, and cell proliferation versus apoptosis, both in vivo and in vitro, and have allowed analysis of the site of response to antigens in vivo.