CLONING AND EXPRESSION OF THE EP2 SUBTYPE OF HUMAN RECEPTORS FOR PROSTAGLANDIN-E2
CLONING AND EXPRESSION OF THE EP2 SUBTYPE OF HUMAN RECEPTORS FOR PROSTAGLANDIN-E2
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DOI：
10.1006/bbrc.1993.2470
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发表时间：
1993-11-30
影响因子：
3.1
通讯作者：
GOETZL, EJ
中科院分区：
文献类型：
--
作者：
AN, SZ;YANG, JH;GOETZL, EJ
前列腺素E2（PGE 2）是许多人体组织中的有效介质，其由三种不同的受体亚型识别，命名为EP 1、EP 2和EP 3。来自人肺文库的cDNA编码与小鼠EP 2受体具有88%同源性的53 kDa蛋白。COS-7细胞转染子中的人EP 2受体以2.2 nM的平均Kd和天然特异性结合[3 H]-PGE 2，并且转导的环AMP的细胞内浓度增加，但不是Ca++。EP 2受体mRNA主要分布于肺、肾、肠、腺和免疫组织中，与功能反应一致。
Prostaglandin E2 (PGE2) is a potent mediator in many human tissues, that is recognized by three distinct subtypes of receptors, designated EP1, EP2 and EP3. A cDNA from a human lung library encodes a 53 kDa protein of 88% homology with the mouse EP2 receptor. Human EP2 receptors in COS-7 cell transfectants bound [3H]-PGE2 with a mean Kd of 2.2 nM and native specificity, and transduced increases in the intracellular concentration of cyclic AMP, but not of Ca++. That most EP2 receptor mRNA is in lung, kidney, intestinal, glandular and immune tissues, is consistent with functional responses.