Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons.
Velocity of conduction between columns and layers in barrel cortex reported by parvalbumin interneurons.
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小清蛋白中间神经元报告的桶状皮层柱和层之间的传导速度。
DOI：
10.1093/cercor/bhad254
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Jackson,MeyerB
中科院分区：
文献类型：
--
作者：
Scheuer,KatherineS;Judge,JohnM;Zhao,Xinyu;Jackson,MeyerB
表达小清蛋白（PV）的抑制性中间神经元在整个大脑中起着关键作用。它们的快速尖峰使它们能够在毫秒的时间尺度上控制电路动态，并且它们通过不同兴奋性通路激活的时间对这些功能至关重要。我们使用了一种遗传编码的混合电压传感器，以亚毫秒的精度在成年小鼠的初级体感桶皮层（BC）的PV中间神经元电压变化的图像。电刺激诱发去极化，其潜伏期随着与刺激电极的距离而增加，使我们能够确定传导速度。皮质层之间的传播反应产生层间传导速度和层内传播产生不同层的层内传导速度。速度范围为74至473 μm/ms，取决于轨迹;层间传导比层内传导快71%。因此，列内的计算比列间的计算更快。BC整合了丘脑和皮质内输入的功能，如纹理辨别和感觉调节。层内和层间PV中间神经元激活之间的时间差异可能会影响这些功能。PV中间神经元的电压成像揭示了皮质回路内信号动力学的差异。这种方法提供了一个独特的机会，调查传导的轴突群体的基础上，他们的靶向特异性。
Inhibitory interneurons expressing parvalbumin (PV) play critical roles throughout the brain. Their rapid spiking enables them to control circuit dynamics on a millisecond time scale, and the timing of their activation by different excitatory pathways is critical to these functions. We used a genetically encoded hybrid voltage sensor to image PV interneuron voltage changes with sub-millisecond precision in primary somatosensory barrel cortex (BC) of adult mice. Electrical stimulation evoked depolarizations with a latency that increased with distance from the stimulating electrode, allowing us to determine conduction velocity. Spread of responses between cortical layers yielded an interlaminar conduction velocity and spread within layers yielded intralaminar conduction velocities in different layers. Velocities ranged from 74 to 473 μm/ms depending on trajectory; interlaminar conduction was 71% faster than intralaminar conduction. Thus, computations within columns are more rapid than between columns. The BC integrates thalamic and intracortical input for functions such as texture discrimination and sensory tuning. Timing differences between intra- and interlaminar PV interneuron activation could impact these functions. Imaging of voltage in PV interneurons reveals differences in signaling dynamics within cortical circuitry. This approach offers a unique opportunity to investigate conduction in populations of axons based on their targeting specificity.