Isolation and characterization of microsatellite loci from three cryptic species of Ceratosolen emarginatus
Isolation and characterization of microsatellite loci from three cryptic species of Ceratosolen emarginatus
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DOI：
10.1111/ens.12179
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发表时间：
2016-07
影响因子：
0.9
通讯作者：
Li-Yuan Yang;Hui-Mei Yu;Da‐Yong Zhang;Wan‐Jin Liao
中科院分区：
文献类型：
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作者：
Li-Yuan Yang;Hui-Mei Yu;Da‐Yong Zhang;Wan‐Jin Liao
From an enriched (AG)n/(AC)n library, we developed nine polymorphic microsatellite loci for three cryptic species of the fig‐pollinating wasp Ceratosolen emarginatus. We genotyped one population for each of the three cryptic species across all the nine loci. In total, 204 alleles were detected from the three cryptic species of C. emarginatus. The observed heterozygosity was 0.755 ± 0.034, 0.653 ± 0.030 and 0.603 ± 0.073 in C. emarginatus populations A, B and C, respectively; the expected heterozygosity was 0.850 ± 0.031, 0.724 ± 0.035 and 0.702 ± 0.104, respectively. No linkage disequilibrium was found between any two loci of all three cryptic species. The newly isolated microsatellite markers will be very useful for estimating the genetic variation within and among the cryptic species and for revealing the mechanisms of speciation and inbreeding coexistence hypothesis of the cryptic species.
