Cytotoxicity of ophthalmic preservatives.
Cytotoxicity of ophthalmic preservatives.
复制标题
眼用防腐剂的细胞毒性。
DOI：
10.1016/0002-9394(74)90016-6
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发表时间：
1974
影响因子：
4.2
通讯作者：
Thomas Miller
中科院分区：
文献类型：
--
作者：
A. Gasset;Yasuo Ishii;Herbert E. Kaufman;Thomas Miller
材料与方法本研究使用了三种常用的防腐剂：苯扎氯铵、氯丁醇和硫柳汞。苯扎氯铵、氯丁醇和硫柳汞的水溶液，浓度分别为4、2、1和0.1%，通过将溶质溶解在正常pH值的蒸馏水中来制备。每只家兔的每只眼睛每天两次接受1滴浓度为4、2、1或0.1%的苯扎氯铵、氯丁醇或硫柳汞。在不同的时间间隔检查眼睛并拍照。在应用这些溶液后1 - 7天处死家兔。在靠近角巩膜利姆布斯处开一个小口以使固定剂更快进入后，将眼睛置于溶于10%中性缓冲福尔马林的0.5%氯化十六烷基吡啶中24小时。然后切除角膜，包括一小圈巩膜，并将其放回相同的固定剂中至少72小时。标本经乙醇脱水后，石蜡包埋，行垂直和水平切片。每只动物的一只眼睛
MATERIALS AND METHODSThe three commonly used preservatives, benzalkonium chloride, chlorobutanol, and thimerosal, were used in this study. Aqueous solutions of benzalkonium chlo ride, chlorobutanol, and thimerosal, in con centrations of 4, 2, 1, and 0.1%, were pre pared by dissolving the solute in distilled water at a normal pH. There were 12 groups of nine rabbits each. Twice a day each rabbit received in each eye one drop of benzalkonium chloride, chloro butanol, or thimerosal in concentrations of 4, 2, 1, or 0.1%. The eyes were examined and photographed at different time intervals. The rabbits were killed one to seven days after the application of these solutions. After a small opening was made near the corneoscleral limbus to permit more rapid access of the fixative, the eye was placed in 0.5% cetylpyridinium chloride in 10% neu tral-buffered formalin for 24 hours. The cornea, including a small ring of sclera, was then excised and returned to the same fixa tive for a minimum of 72 hours. After ethanol dehydration, the specimens were em bedded in paraffin for perpendicular and hor izontal sectioning. An eye from each animal