Monitoring of cAMP synthesis and degradation in living cells
Monitoring of cAMP synthesis and degradation in living cells
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DOI：
10.1152/physiol.00057.2005
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发表时间：
2006-04-01
期刊：
影响因子：
8.4
通讯作者：
Lohse, MJ
中科院分区：
文献类型：
--
作者：
Nikolaev, VO;Lohse, MJ
cAMP是一种重要的第二信使，具有多种细胞效应和生物学作用。为了监测和可视化完整活细胞中的cAMP，已经开发了基于所有三种类型的cAMP效应器的激活的电生理和荧光方法：蛋白激酶A、环核苷酸门控通道和由cAMP直接激活的交换蛋白。在这篇综述中，我们描述和比较这些技术的鲁棒性，灵敏度和时空分辨率。
cAMP is an important second messenger with a plethora of cellular effects and bio-logical roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleoticle-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.