Vitamin B12 bioaccumulation in Chlorella vulgaris grown on food waste-derived anaerobic digestate
Vitamin B12 bioaccumulation in Chlorella vulgaris grown on food waste-derived anaerobic digestate
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DOI：
10.1016/j.algal.2023.103290
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发表时间：
2023-10-19
影响因子：
5.1
通讯作者：
Mehrshahi，Payam
中科院分区：
文献类型：
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作者：
Papadopoulos，Konstantinos P.;de Souza，Marcella Fernandes;Mehrshahi，Payam
厌氧消化在现代循环经济中发挥着关键作用，因为它为将有机废物转化为沼气（甲烷）提供了可持续的解决方案。它还会产生营养丰富的液体流，称为消化物。由于其常量营养素（N、P）含量高，这种沼渣被广泛用作农田肥料，但其所含的生物活性微量营养素及其对下游应用的意义仍然很大程度上未知。在这里，我们研究了缺乏维生素 B12 的底物（水果和蔬菜废物）产生的消化物是否可以通过厌氧消化来富集这种维生素，并探索微藻普通小球藻在这种培养基中生长和生物富集 B12 的能力。我们的研究结果首次发现，厌氧消化过程中会合成大量 B12，并且 C.vulgaris 在消化物中生长时可以有效地富含这种维生素（10.6 µg Β12·g−1 干重）。此外，我们发现 pH 引起的氨毒性是在消化物中培养微藻时的主要抑制剂，这使我们能够通过 pH 控制显着提高实验室和中试规模的生产力。消化物中 B12 的合成和微藻中积累的案例凸显了通过识别和利用生物活性化合物来提高这些废物流的价值的潜力。
Anaerobic digestion plays a pivotal role in the modern circular economy, as it offers a sustainable solution for converting organic waste into biogas (methane). It also results in a nutrient-rich liquid stream, referred to as digestate. This digestate is extensively applied to agricultural land as fertilizer due to its high macronutrient (N, P) content, but the bioactive micronutrients it contains and their significance for downstream applications remain largely unknown. Here, we investigate whether digestate generated from a vitamin B12-deficient substrate (fruit and vegetable waste) can be enriched in this vitamin through anaerobic digestion, and explore the capability of the microalgaChlorella vulgaristo grow in this medium and bioaccumulate B12. Our findings uncover, for the first time, that substantial amounts of B12are synthesized during anaerobic digestion, and thatC.vulgariscan effectively be enriched with this vitamin when grown in the digestate (10.6 μg Β12·g−1dry weight). Additionally, we identified that pH-induced ammonia toxicity was the main inhibitor when growing microalgae in the digestate, which allowed us to significantly enhance productivity at lab- and pilot-scale through pH control. The case of B12synthesis in digestate and accumulation in microalgae highlights the potential for enhancing the value of these waste streams through the identification and utilization of bioactive compounds.