Fibroblast growth factor (FGF) homologous factors: New members of the FGF family implicated in nervous system development
Fibroblast growth factor (FGF) homologous factors: New members of the FGF family implicated in nervous system development
复制标题
DOI：
10.1073/pnas.93.18.9850
复制
发表时间：
1996-09-03
影响因子：
11.1
通讯作者：
Nathans, J
中科院分区：
文献类型：
--
作者：
Smallwood, PM;MunozSanjuan, I;Nathans, J
成纤维细胞生长因子 (FGF) 家族的四个新成员，称为成纤维细胞生长因子同源因子 (FHF)，已通过随机 cDNA 测序、数据库搜索和简并 PCR 的组合鉴定出。四个 FHF 之间的配对比较显示出 58% 至 71% 的氨基酸序列同一性，但每个 FHF 与其他 FGF 相比显示出不到 30% 的同一性。与 FGF-1（酸性 FGF）和 FGF-2（碱性 FGF）一样，FHF 缺乏经典信号序列，并且包含可充当核定位信号的碱性残基簇。在瞬时转染的 293 细胞中，FHF-1 在细胞核中积聚并且不分泌。每个 FHF 在发育中和成人神经系统中表达，表明 FGF 家族的这一分支在神经系统发育和功能中发挥作用。
Four new members of the fibroblast growth factor (FGF) family, referred to as fibroblast growth factor homologous factors (FHFs), have been identified by a combination of random cDNA sequencing, data base searches, and degenerate PCR Pairwise comparisons between the four FHFs show between 58% and 71% amino acid sequence identity, but each FHF shows less than 30% identity when compared with other FGFs. Like FGF-1 (acidic FGF) and FGF-2 (basic FGF), the FHFs lack a classical signal sequence and contain clusters of basic residues that can act as nuclear localization signals. In transiently transfected 293 cells FHF-1 accumulates in the nucleus and is not secreted. Each FHF is expressed in the developing and adult nervous systems, suggesting a role for this branch of the FGF family in nervous system development and function.