Rapid transcriptional plasticity of duplicated gene clusters enables a clonally reproducing aphid to colonise diverse plant species.
Rapid transcriptional plasticity of duplicated gene clusters enables a clonally reproducing aphid to colonise diverse plant species.
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DOI：
10.1186/s13059-016-1145-3
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发表时间：
2017-02-13
期刊：
影响因子：
12.3
通讯作者：
Hogenhout SA
中科院分区：
文献类型：
--
作者：
Mathers TC;Chen Y;Kaithakottil G;Legeai F;Mugford ST;Baa-Puyoulet P;Bretaudeau A;Clavijo B;Colella S;Collin O;Dalmay T;Derrien T;Feng H;Gabaldón T;Jordan A;Julca I;Kettles GJ;Kowitwanich K;Lavenier D;Lenzi P;Lopez-Gomollon S;Loska D;Mapleson D;Maumus F;Moxon S;Price DR;Sugio A;van Munster M;Uzest M;Waite D;Jander G;Tagu D;Wilson AC;van Oosterhout C;Swarbreck D;Hogenhout SA
宿主-寄生虫进化的主流范式是，军备竞赛通过遗传适应导致日益专业化。昆虫食草动物也不例外，它们中的大多数进化为定居在少数密切相关的寄主物种上。值得注意的是，桃长管蚜寄生在40个科的植物物种上，单个桃蚜无性系谱系可以定殖远缘植物。这种非凡的能力使桃小实蝇成为许多重要农作物的极具破坏性的害虫。为了研究烟粉虱特殊的表型可塑性，我们对烟粉虱基因组进行了测序，并评估了一个克隆谱系对不同科的寄主植物物种的反应。我们发现，基因相同的个体能够通过对属于蚜虫扩展基因家族的基因的不同调控来殖民远亲寄主物种。在寄主转换后的两天内，多基因簇在单一的蚜虫体内集体上调。此外，我们使用RNA干扰(RNAi)介导的组织蛋白酶B基因家族基因的敲除来证明这种快速转录变化的功能意义。组织蛋白酶B基因的敲除降低了蚜虫的适合度，但仅限于诱导这些基因上调的寄主。此前的研究主要集中在寄生虫对宿主的遗传适应作用上。在这里，我们表明，多面手的桃蚜害虫能够在没有遗传专门化的情况下定居在不同的寄主植物物种上。这是通过在蚜虫进化过程中复制的基因的快速转录可塑性实现的。本文的在线版本(doi：10.1186/s13059-0161145-3)包含补充材料，授权用户可以使用。
The prevailing paradigm of host-parasite evolution is that arms races lead to increasing specialisation via genetic adaptation. Insect herbivores are no exception and the majority have evolved to colonise a small number of closely related host species. Remarkably, the green peach aphid, Myzus persicae, colonises plant species across 40 families and single M. persicae clonal lineages can colonise distantly related plants. This remarkable ability makes M. persicae a highly destructive pest of many important crop species. To investigate the exceptional phenotypic plasticity of M. persicae, we sequenced the M. persicae genome and assessed how one clonal lineage responds to host plant species of different families. We show that genetically identical individuals are able to colonise distantly related host species through the differential regulation of genes belonging to aphid-expanded gene families. Multigene clusters collectively upregulate in single aphids within two days upon host switch. Furthermore, we demonstrate the functional significance of this rapid transcriptional change using RNA interference (RNAi)-mediated knock-down of genes belonging to the cathepsin B gene family. Knock-down of cathepsin B genes reduced aphid fitness, but only on the host that induced upregulation of these genes. Previous research has focused on the role of genetic adaptation of parasites to their hosts. Here we show that the generalist aphid pest M. persicae is able to colonise diverse host plant species in the absence of genetic specialisation. This is achieved through rapid transcriptional plasticity of genes that have duplicated during aphid evolution. The online version of this article (doi:10.1186/s13059-016-1145-3) contains supplementary material, which is available to authorized users.