Metabolic pathway analysis: Basic concepts and scientific applications in the post-genomic era
Metabolic pathway analysis: Basic concepts and scientific applications in the post-genomic era
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DOI：
10.1021/bp990048k
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发表时间：
1999-05-01
影响因子：
2.9
通讯作者：
Heinrich, R
中科院分区：
文献类型：
--
作者：
Schilling, CH;Schuster, S;Heinrich, R
本文回顾了代谢途径分析的相对较短的历史。计算机辅助算法的合成代谢途径进行了讨论。对通路分析中用到的重要代数概念，如零空间、凸锥等进行了解释。它演示了如何将这些概念转化为有意义的代谢概念。例如，它表明，最简单的矢量跨越区域的所有容许通量在静止状态下，其中的术语基本通量模式被创造出来，对应于系统中的基本路径。这些概念说明的帮助下的乙醛酸循环和相邻的天冬氨酸和谷氨酸合成反应的反应方案。途径分析和代谢控制理论之间的相互关系进行了概述。有前途的应用基因组注释和生物技术的目的进行了讨论。有了对细胞代谢结构的更好理解和当今可用的大量基因组数据，生物化学家和生物技术专家将能够绘制细胞的整个代谢图，并通过合理和定向的代谢工程对其进行重新设计。
This article reviews the relatively short history of metabolic pathway analysis. Computer-aided algorithms for the synthesis of metabolic pathways are discussed. Important algebraic concepts used in pathway analysis, such as null space and convex cone, are explained. It is demonstrated how these concepts can be translated into meaningful metabolic concepts. For example, it is shown that the simplest vectors spanning the region of all admissible fluxes in stationary states, for which the term elementary flux modes was coined, correspond to fundamental pathways in the system. The concepts are illustrated with the help of a reaction scheme representing the glyoxylate cycle and adjacent reactions of aspartate and glutamate synthesis. The interrelations between pathway analysis and metabolic control theory are outlined. Promising applications for genome annotation and for biotechnological purposes are discussed. Armed with a better understanding of the architecture of cellular metabolism and the enormous amount of genomic data available today, biochemists and biotechnologists will be able to draw the entire metabolic map of a cell and redesign it by rational and directed metabolic engineering.