USE OF METRONIDAZOLE AS A PROBE IN THE STUDY OF HUMAN PERIODONTAL-DISEASE
USE OF METRONIDAZOLE AS A PROBE IN THE STUDY OF HUMAN PERIODONTAL-DISEASE
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DOI：
10.1111/j.1600-051x.1983.tb01271.x
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发表时间：
1983-01-01
影响因子：
6.7
通讯作者：
BORJESSON, I
中科院分区：
文献类型：
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作者：
LINDHE, J;LILJENBERG, B;BORJESSON, I
关于人类受试者炎症性牙周病的持续存在与牙龈下微生物群的厌氧部分的消除是不相容的假设进行了测试。16名人类受试者参加了试验。他们是根据已经产生至少4对对侧牙齿的晚期牙周病来选择的。在基线检查时，病变部位的特征是评估菌斑、牙龈炎、探探深度和附着水平。此外，还测定了龈下微生物群的组成。对其中一个部位进行活检。在组织样本中，评估炎症细胞浸润的大小。在基线检查后，将患者随机分为2个治疗组，实验组和对照组。试验组患者给予甲硝唑治疗，疗程为3期，每期2周，间隔8周。此外，所有患者都接受了详细的口腔卫生指导，并进行了包括左颌和右颌2象限牙列的一系列龈下洗刷。在基线检查后2、10、20、30和50周复查。在治疗开始后2、20和50周进行复查时，从预定部位进行活检。从牙龈下微生物群中消除甲硝唑敏感细菌导致牙周病的临床和组织病理学症状消失。甲硝唑对厌氧菌具有独特的杀菌范围。因此，目前的研究结果强调了牙龈下菌斑的厌氧部分在牙周病炎症成分维持中所起的核心作用。
The hypothesis that the persistence of inflammatory periodontal disease in human subjects is incompatible with the elimination of the anaerobic segments of the subgingival microbiota was tested. Sixteen human subjects participated in the trial. They were selected on the basis of advanced periodontal disease which had produced at least 4 pairs of contralateral teeth with advanced disease. At the baseline examination the diseased sites were characterized by assessment of plaque, gingivitis, probing depth and attachment level. In addition, the composition of the subgingival microbiota was determined. One pair of sites was subjected to biopsy. In the tissue samples the size of the inflammatory cell infiltrate was assessed. Following the baseline examination the patients were randomly distributed into 2 treatment groups, test and control. The patients of the test group received metronidazole for 3 periods of 2 wk each, separated by intervals of 8 wk. In addition, all patients received detailed oral hygiene instruction and a series of subgingival scalings involving 2 quadrants of the dentition, either the right or the left jaw quadrants. Reexaminations were performed 2, 10, 20, 30 and 50 wk after the baseline examination. Biopsies from the predetermined sites were obtained at the reexaminations performed 2, 20 and 50 wk after the initiation of the therapy. The elimination of metronidazole-sensitive bacteria from the subgingival microbiota resulted in the disappearance of clinical and histopathological signs of periodontal disease. Metronidazole has a unique bactericidal range against anaerobic bacteria. The present findings, therefore, emphasize the central role played by the anaerobic segments of the subgingival plaque for the maintenance of the inflammatory component of periodontal disease.