THE MOLECULAR-GENETICS OF ENHANCER OF SPLIT, A GENE REQUIRED FOR EMBRYONIC NEURAL DEVELOPMENT IN DROSOPHILA
THE MOLECULAR-GENETICS OF ENHANCER OF SPLIT, A GENE REQUIRED FOR EMBRYONIC NEURAL DEVELOPMENT IN DROSOPHILA
复制标题
DOI：
10.1002/j.1460-2075.1988.tb03278.x
复制
发表时间：
1988-12-01
期刊：
影响因子：
11.4
通讯作者：
ARTAVANISTSAKONAS, S
中科院分区：
文献类型：
--
作者：
PREISS, A;HARTLEY, DA;ARTAVANISTSAKONAS, S
在果蝇中，神经发生的最初步骤似乎是由一小群称为神经发生位点的合子作用基因控制的。这些基因中的任何一个突变都会导致表皮谱系错误地进入神经通路。形态学和分子研究表明，正确的外胚层分化是由细胞间相互作用机制介导的，并且至少一些神经源性位点参与了该机制。对神经源性位点Notch和Delta的分子分析表明，假定的基因产物是与哺乳动物表皮生长因子同源的大跨膜蛋白。我们在此描述了对分裂增强子 [E(spl)] 的分子分析，这是第三个神经源性位点，它显示出与 Notch 和 Delta 的显着遗传相互作用，表明各自基因产物的密切功能关系。我们提供了对应于野生型 E(spl) 功能所必需的单个转录单位的单个遗传互补组的证据。 P元件介导的转化表明该转录单位包括与显性E(spl)D突变和隐性可见等位基因groucho相关的功能，并且对于胚胎神经系统的正确分化是必需的。
In Drosophila, the very first steps in neurogenesis appear to be controlled by a small group of zygotically acting genes termed the neurogenic loci. Mutations in any of these genes result in a misrouting of epidermal lineages into the neural pathway. Morphological and molecular studies suggest that the correct ectodermal differentiation is mediated by a cell-cell interaction mechanism and that at least some of the neurogenic loci are involved in this mechanism. The molecular analyses of the neurogenic loci Notch and Delta revealed that the putative gene products are large transmembrane proteins with homology to mammalian epidermal growth factor. We describe here a molecular analysis of Enhancer of split [E(spl)], a third neurogenic locus, which displays striking genetic interactions with both Notch and Delta, suggesting a close functional relationship of the respective gene products. We provide evidence for a single genetic complementation group corresponding to a single transcription unit which is necessary for wild-type E(spl) function. P-element-mediated transformation indicates that this transcription unit includes functions associated with both the dominant E(spl)D mutation and the recessive visible allele groucho, and is necessary for the correct differentiation of the embryonic nervous system.