Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ.
Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ.
复制标题
小鼠牙胚分化过程中基质蛋白、纤连蛋白和胶原蛋白的变化。
DOI：
10.1016/0012-1606(79)90011-3
复制
发表时间：
1979
影响因子：
2.7
通讯作者：
R. Timpl
中科院分区：
文献类型：
--
作者：
Irma Thesleff;S. Stenman;Antti Vaheri;R. Timpl
The distribution of the matrix protein fibronectin was studied by indirect immunofluorescence in differentiating mouse molars from bud stage to the stage of dentin and enamel secretion, and compared to that of collagenous proteins procollagen type III and collagen type I. Fibronectin was seen in mesenchymal tissue, basement membranes, and predentin. The dental mesenchyme lost fibronectin staining when differentiating into odontoblasts. Fibronectin was not detected in mineralized dentin. Epithelial tissues were negative except for the stellate reticulum within the enamel organ. Particularly intense staining was seen at the epithelio-mesenchymal interface between the dental epithelium and mesenchyme. Fibronectin may here be involved in anchorage of the mesenchymal cells during their differentiation into odontoblasts. Procollagen type III was lost from the dental mesenchyme during odontoblast differentiation but reappeared with advancing vascularization of the dental papilla. Similarly, procollagen type III present in the dental basement membrane during the bud and cap stages disappeared from the cuspal area along with odontoblast differentiation. Weak staining was seen in predentin but not in mineralized dentin. The staining with anti-collagen type I antibodies was weak in dental mesenchyme but intense in predentin as well as in mineralized dentin.
