Generating gene knockout rats by homologous recombination in embryonic stem cells.
Generating gene knockout rats by homologous recombination in embryonic stem cells.
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通过胚胎干细胞同源重组产生基因敲除大鼠。
DOI：
10.1038/nprot.2011.338
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发表时间：
2011-06
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
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作者：
We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and expansion of rat ES cells, construction of gene-targeting vectors, generation of gene-targeted rat ES cells and, finally, production of gene-targeted rats. The major differences between this protocol and the classical mouse gene-targeting protocol include ES cell culture methods, drug selection scheme, colony picking and screening strategies. This ES cell–based gene-targeting technique allows sophisticated genetic modifications to be performed in the rat, as many laboratories have been doing in the mouse for the past two decades. Recently we used this protocol to generate Tp53 (also known as p53) gene knockout rats. The entire process requires ~1 year to complete, from derivation of ES cells to generation of knockout rats.
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影响因子：
2.7
作者：
Auerbach, W;Dunmore, JH;Joyner, AL
通讯作者：
Joyner, AL
DOI：
10.1073/pnas.1009424107
发表时间：
2010-08-24
影响因子：
11.1
作者：
Meyer, Melanie;de Angelis, Martin Hrabe;Kuehn, Ralf
通讯作者：
Kuehn, Ralf
影响因子：
64.5
作者：
Li P;Tong C;Mehrian-Shai R;Jia L;Wu N;Yan Y;Maxson RE;Schulze EN;Song H;Hsieh CL;Pera MF;Ying QL
通讯作者：
Ying QL
DOI：
10.1073/pnas.0604657103
发表时间：
2006-07-25
影响因子：
11.1
作者：
Dann, Christina Tenenhaus;Alvarado, Alma L.;Garbers, David L.
通讯作者：
Garbers, David L.
DOI：
10.1126/science.1172447
发表时间：
2009-07-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Geurts AM;Cost GJ;Freyvert Y;Zeitler B;Miller JC;Choi VM;Jenkins SS;Wood A;Cui X;Meng X;Vincent A;Lam S;Michalkiewicz M;Schilling R;Foeckler J;Kalloway S;Weiler H;Ménoret S;Anegon I;Davis GD;Zhang L;Rebar EJ;Gregory PD;Urnov FD;Jacob HJ;Buelow R
通讯作者：
Buelow R