Host phylogeny and ecological associations best explain Wolbachia host shifts in scale insects
Host phylogeny and ecological associations best explain Wolbachia host shifts in scale insects
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DOI：
10.1111/mec.16883
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发表时间：
2023-03-03
影响因子：
4.9
通讯作者：
Engelstadter，Jan
中科院分区：
文献类型：
--
作者：
Sanaei，Ehsan;Albery，Gregory F.;Engelstadter，Jan
沃尔巴克氏体是地球上最普遍和最广泛的内共生细菌之一。沃尔巴克氏体成功感染大量节肢动物物种归因于两个特征：它们在宿主中诱导的表型范围，以及它们在宿主物种之间转换的能力。虽然在阐明其诱导表型方面取得了很大进展，但我们对Wolbachia宿主转移的理解仍然非常有限：我们甚至缺乏关于Wolbachia转移途径和影响宿主转移的因素的重要性的基本问题的答案。在这里，我们调查了沃尔巴克氏体蚧虫的多样性和宿主转移模式，这是一组与其他昆虫密切相关的节肢动物，使它们非常适合研究宿主转移。使用Illumina多靶扩增子测序沃尔巴克氏体感染的蚧虫及其直接联系人，我们确定了所有沃尔巴克氏体菌株的身份。然后，我们拟合了一个广义的加性混合模型，我们的数据来估计的影响，主机的生殖和地理分布对沃尔巴克氏体菌株共享的蚧虫物种。该模型预测宿主地理没有显著贡献，但宿主系统发育有很强的影响，在密切相关的物种中，Wolbachiasharing的比率很高，随着系统发育距离的增加，共享率突然下降。我们还在介壳虫和几种密切相关的物种（蚂蚁、黄蜂和苍蝇）中检测到了相同的沃尔巴克氏体菌株。这表明了假定的主机转移和潜在的转移途径，通过这些联营公司，并强调了在沃尔巴克氏体主机转移生态连接的重要性。
Wolbachiaare among the most prevalent and widespread endosymbiotic bacteria on Earth.Wolbachia's success in infecting an enormous number of arthropod species is attributed to two features: the range of phenotypes they induce in their hosts, and their ability to switch between host species. Whilst much progress has been made in elucidating their induced phenotypes, our understanding ofWolbachiahost‐shifting is still very limited: we lack answers to even fundamental questions concerningWolbachia's routes of transfer and the importance of factors influencing host shifts. Here, we investigate the diversity and host‐shift patterns ofWolbachiain scale insects, a group of arthropods with intimate associations with other insects that make them well suited to studying host shifts.Using Illumina multitarget amplicon sequencing ofWolbachia‐infected scale insects and their direct associates we determined the identity of allWolbachiastrains. We then fitted a generalized additive mixed model to our data to estimate the influence of host phylogeny and the geographical distribution onWolbachiastrain sharing among scale insect species. The model predicts no significant contribution of host geography but strong effects of host phylogeny, with high rates ofWolbachiasharing among closely related species and a sudden drop‐off in sharing with increasing phylogenetic distance. We also detected the sameWolbachiastrain in scale insects and several intimately associated species (ants, wasps and flies). This indicates putative host shifts and potential routes of transfers via these associates and highlights the importance of ecological connectivity inWolbachiahost‐shifting.