Postnatal Msx1 expression pattern in craniofacial, axial, and appendicular skeleton of transgenic mice from the first week until the second year
Postnatal Msx1 expression pattern in craniofacial, axial, and appendicular skeleton of transgenic mice from the first week until the second year
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DOI：
10.1002/dvdy.1120
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发表时间：
2001-05-01
影响因子：
2.5
通讯作者：
Berdal, A
中科院分区：
文献类型：
--
作者：
Orestes-Cardoso, SM;Nefussi, JR;Berdal, A
与Msx1突变相关的表型已经确立了这种不同的同源基因在骨骼模式中的突出作用。以前的研究已经完成了在产前发育与零突变小鼠早期死亡的关系。因此，本研究致力于研究Msx1同源基因在出生后的头面部、中枢骨和附件骨中的表达。从出生后第一周到15个月，研究了一个敲入转基因小鼠品系。整装β-半乳糖苷酶鉴定了Msx1蛋白的表达模式。在生长期和成年小鼠中观察到Msx1的持续表达，特别是在Msx1在最初的骨骼图案形成过程中发挥早期形态发生作用的位置。这些包括颅面缝、自体平台、下颌骨和牙槽骨。此外，活跃的膜和软骨内骨形成涉及整个骨骼的Msx1。组织切片显示，所有骨细胞系的祖细胞以及分化和分化的细胞都能表达Msx1蛋白(软骨细胞、成骨细胞、抗酒石酸酸性磷酸酶阳性破骨细胞和软骨破骨细胞)。骨骼形态发生的遗传和发育生物学的最新进展表明，发育关键基因在离散的胚胎信号和生长中心、牙齿的釉质结、颅骨形态发生的颅缝和肢体芽的进展区共同表达。本研究表明，这些信号通路在整个生命过程中共同重要，具有与早期图案相关的精细的位置和空间特异性。(C)2001年Wiley-Liss，Inc.
Phenotypes associated with Msx1 mutations have established the prominent role of this divergent homeogene in skeletal patterning. Previous studies have been achieved during antenatal development in relation with the early death of null mutant mice. Therefore, the present study is devoted to Msx1 homeogene in the postnatal craniofacial, axial, and appendicular skeleton. A knock-in transgenic mouse line was studied from the first postnatal week until 15 months. Whole-mount beta -galactosidase enzymology identified Msx1 protein expression pattern. Maintained expression of Msx1 was observed in growing and adult mice, specifically in the sites where Msx1 plays an early morphogenetic role during initial skeletal patterning. These included the craniofacial sutures, autopodium, mandible, and alveolar bone. Furthermore, active membranous and endochondral bone formation involved Msx1 in the entire skeleton. Histologic sections showed that progenitor as well as differentiating and differentiated cells of all the bone cell lineages could express the Msx1 protein (chondrocytes, osteoblasts, tartrate-resistant acid phosphatase positive osteoclasts and chondroclasts), Recent developments in the genetic and developmental biology of skeletal morphogenesis demonstrate that genes critical for development are jointly expressed in discrete embryonic signalling and growth centers, the enamel knot in teeth, the cranial suture in skull morphogenesis, and the progress zone in the limb buds, The present study suggests that these signalling pathways are jointly important throughout the entire lifetime with an exquisite site-specificity spatially related to early patterning. (C) 2001 Wiley-Liss, Inc.