Spontaneous Interconversion between Different Narrowly Defined Shapes of Rotavirus Double-Layered Particles Studied in Real Time by High-Resolution Mobility Analysis.
Spontaneous Interconversion between Different Narrowly Defined Shapes of Rotavirus Double-Layered Particles Studied in Real Time by High-Resolution Mobility Analysis.
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通过高分辨率迁移率分析实时研究轮状病毒双层颗粒不同狭窄定义形状之间的自发相互转化。
DOI：
10.1021/acs.analchem.3c01994
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发表时间：
2023
影响因子：
7.4
通讯作者：
Javier M. Rodríguez
中科院分区：
文献类型：
--
作者：
J. Fernández de la Mora;F. Almazán;Javier M. Rodríguez
用高分辨率微分迁移率分析仪（DMA）在气相中研究轮状病毒双层颗粒（DLP）。将DLP转移到10 mM乙酸铵水溶液中，电喷雾到气相中，转化成主要带单电荷的颗粒，并进行DMA分析。多达七个略有不同的构象得到解决，其明显的随机，快速（分钟），可逆的相互转化遵循真实的时间。它们有时演变成两种不同的结构，一种结构支配另一种结构，反之亦然。溶液和气相中DLP结构之间的差异通过此处发现的较小DLP直径（60对70 nm）清楚地揭示。然而，我们认为，多个气相构象观察到起源于许多构象预先存在于解决方案中。我们进一步假设，这些构象对应于不完整的DLP已经失去了一些周围的12个5倍轴的VP6三聚体五联体。先前已经通过冷冻电子显微镜对轮状病毒Wa株以及RotaTec疫苗中包含的其他五种轮状病毒株的电荷检测质谱法记录了这种特殊损失的90%。迄今为止在醋酸铵水溶液中研究的所有7种轮状病毒类型都系统地发现了这种损失的证据，这可能是这种电解质的一个特殊特征。
Rotavirus double-layered particles (DLPs) are studied in the gas phase with a high-resolution differential mobility analyzer (DMA). DLPs were transferred to 10 mM aqueous ammonium acetate, electrosprayed into the gas phase, converted into primarily singly charged particles, and DMA-analyzed. Up to seven slightly different conformations were resolved, whose apparently random, fast (minutes), and reversible interconversions were followed in real time. They sometimes evolved into just two distinct structures, with periods of one dominating over the other and vice versa. Differences between the DLP structures in solution and in the gas phase are clearly revealed by the smaller DLP diameter found here (60 versus 70 nm). Nevertheless, we argue that the multiple gas-phase conformers observed originate in as many conformations pre-existing in solution. We further hypothesize that these conformers correspond to incomplete DLPs having lost some of the VP6 trimer quintets surrounding each of the 12 5-fold axes. Instances of this peculiar loss have been previously documented by cryoelectron microscopy for the rotavirus Wa strain, as well as via charge detection mass spectrometry for five other rotavirus strains included in the RotaTec vaccine. Evidence of this loss systematically found for all 7 rotavirus types so far studied in aqueous ammonium acetate may be a special feature of this electrolyte.
轮状病毒基因组的可逆缩合和扩展。
DOI：
10.1073/pnas.98.4.1381
发表时间：
2001
影响因子：
11.1
作者：
Pesavento,JB;Lawton,JA;Estes,ME;VenkataramPrasad,BV
通讯作者：
VenkataramPrasad,BV