POSTTRANSLATIONAL MODIFICATION OF POLY(ADP-RIBOSE) POLYMERASE INDUCED BY DNA STRAND BREAKS
POSTTRANSLATIONAL MODIFICATION OF POLY(ADP-RIBOSE) POLYMERASE INDUCED BY DNA STRAND BREAKS
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DOI：
10.1016/s0968-0004(00)89089-1
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发表时间：
1995-10-01
影响因子：
13.8
通讯作者：
KLUNGLAND, A
中科院分区：
文献类型：
--
作者：
LINDAHL, T;SATOH, MS;KLUNGLAND, A
哺乳动物细胞核中有100万个聚腺苷二磷酸核糖聚合酶(PARP)分子，该酶存在于大多数真核生物中，但酵母菌除外。作为对电离辐射或烷化剂造成的DNA损伤的反应，PARP与DNA中的链中断结合，并通过合成高负电荷聚(ADP-核糖)的长支化聚合物进行快速自化。DNA修复发生在修饰的PARP从DNA链断裂中解离后。酶耗竭提取物的生化数据和对酶缺陷小鼠的研究表明，PARP不直接参与DNA修复。讨论了聚腺苷二磷酸核糖合成的可能作用。
There are one million molecules of poly(ADP-ribose) polymerase (PARP) in mammalian cell nuclei and the enzyme is found in most eukaryotes, with the notable exception of yeasts. In response to DNA damage caused by ionizing radiation or alkylating agents, PARP binds to strand interruptions in DNA and undergoes rapid automodification with synthesis of long branched polymers of highly negatively charged poly(ADP-ribose). DNA repair occurs after dissociation of modified PARP from DNA strand breaks. Biochemical data with enzyme-depleted extracts and studies of enzyme-deficient mice show that PARP does not participate directly in DNA repair. Possible roles for poly(ADP-ribose) synthesis are discussed.