Destabilisation of the c-kit1 G-quadruplex structure by N6-methyladenosine modification
Destabilisation of the c-kit1 G-quadruplex structure by N6-methyladenosine modification
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DOI：
10.1016/j.bbrc.2020.01.116
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发表时间：
2020-04-02
影响因子：
3.1
通讯作者：
Yoshida, Wataru
中科院分区：
文献类型：
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作者：
Laddachote, Saowalak;Nagata, Mayu;Yoshida, Wataru
N-6-甲基腺嘌呤(m(6)da)是最近在真核生物基因组DNA中发现的一种新化合物。然而，关于它的生物学作用的报道很少。G-四链体(G4)是一种由G-四分体堆积而成的非规范核酸结构。基因组DNA中的G4形成序列富含顺式调控元件，G4结构在多种细胞功能中具有重要作用。我们以前报道过CpG甲基化稳定了血管内皮生长因子(VEGF)G4结构。在这里，我们报告了m(6)da修饰破坏了人c-kit1G4结构的稳定性。这些结果表明，表观遗传修饰可能影响G4的形成，从而调节其生物学功能。(C)2020 Elsevier Inc.保留所有权利。
N-6-methyladenine (m(6)dA) has been recently discovered in eukaryotic genomic DNA. However, there have been few reports on its biological roles. G-quadruplex (G4) is a non-canonical nucleic acid structure formed by the stacking of G-tetrads. G4-forming sequences are enriched with cis-regulatory elements in genomic DNA and the G4 structures have important roles in various cellular functions. We previously reported that CpG methylation stabilized vascular endothelial growth factor (VEGF) G4 structure. Here we report that m(6)dA modification destabilizes the human c-kit1 G4 structure. These results suggest that epigenetic modifications may affect G4 formation in order to regulate the biological functions. (C) 2020 Elsevier Inc. All rights reserved.