Plasmon-induced hyperthermia: hybrid upconversion NaYF4:Yb/Er and gold nanomaterials for oral cancer photothermal therapy
Plasmon-induced hyperthermia: hybrid upconversion NaYF4:Yb/Er and gold nanomaterials for oral cancer photothermal therapy
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DOI：
10.1039/c5tb01393c
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Liu, Ru-Shi
中科院分区：
文献类型：
--
作者：
Chen, Chieh-Wei;Lee, Po-Han;Liu, Ru-Shi
由上转换纳米粒子(UCPs)和等离子体材料组成的纳米复合材料已被广泛用于生物成像和癌症光热治疗(PTT)。然而，仍然存在一些挑战，包括令人费解的能量转移过程的效率和等离子体诱导光热效应的条件优化。在本研究中，我们制备了NaYF4：Yb3+/Er3+纳米粒子(NPs)与金纳米材料(Au NMS)结合，如Au纳米粒子和金纳米棒(Au NRs)。以NaYF4：Yb3+/Er3+纳米粒子为光转换材料，在980 nm激光激发下发射绿光和红光，并制备了Au纳米粒子和Au Nr纳米粒子作为发热体。硅壳被进一步覆盖在UCPs周围，以提高生物相容性，并作为连接UCPs和Au NMS的桥梁。最重要的是，精确地调节了硅壳层的厚度，以研究等离子体场对热诱导的有效距离。连接到Au NMS后的UCL光致发光和发射衰减时间的下降证实了能量的转移。此外，利用有限元方法建立了模拟模型，比较了UCP@SiO_2-NPs和UCP@SiO_2-NRS在生热方面的差异。杂化纳米复合材料的表面用叶酸修饰，以提高对癌细胞的特异性靶向性。评价了改性杂化纳米复合材料在OECM-1口腔癌细胞PTT中的性能。
Nanocomposites consisting of upconversion nanoparticles (UCPs) and plasmonic materials have been widely explored for bio-imaging and cancer photothermal therapy (PTT). However, several challenges, including incomprehensible efficiency of energy transfer processes and optimization of the conditions for plasmon-induced photothermal effects, still exist. In this study, we fabricated NaYF4:Yb3+/Er3+ nanoparticles (NPs) conjugated with gold nanomaterials (Au NMs), such as Au NPs and gold nanorods (Au NRs). NaYF4: Yb3+/Er3+ NPs were used as photoconverters, which could emit green and red light under excitation of a 980 nm laser; Au NPs and Au NRs were also prepared and used as heat producers. The silica shell was further coated around UCPs to improve biocompatibility and as a bridge linking UCPs and the Au NMs. Most importantly, the thickness of the silica shell was tuned precisely to investigate the effective distance of the plasmonic field for heat induction. Energy transfer was confirmed by the declining UCL photoluminescence and emission decay time after connecting to the Au NMs. Moreover, a simulative model was built using the finite element method to assess the differences in heat generation between UCP@SiO2-NPs and UCP@SiO2-NRs. The surfaces of the hybrid nanocomposites were modified with folic acid to improve the specific targeting to cancer cells. The performance of the modified hybrid nanocomposites in PTT for OECM-1 oral cancer cells was evaluated.