Thermal constraints on in vivo optogenetic manipulations
Thermal constraints on in vivo optogenetic manipulations
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DOI：
10.1038/s41593-019-0422-3
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发表时间：
2019-07-01
影响因子：
25
通讯作者：
Kreitzer, Anatol C.
中科院分区：
文献类型：
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作者：
Owen, Scott F.;Liu, Max H.;Kreitzer, Anatol C.
光遗传学的一个关键假设是光只影响表达视蛋白的神经元。然而，照明总是会加热组织，并且许多生理过程对温度敏感。常用的照明方案使温度升高 0.2-2 摄氏度，并抑制多个大脑区域的尖峰。在纹状体中，光传递激活了内向整流钾电导和偏向旋转行为。因此，需要仔细考虑光传输参数，因为即使是适度的颅内加热也会混淆光遗传学实验的解释。
A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly used illumination protocols increased the temperature by 0.2-2 degrees C and suppressed spiking in multiple brain regions. In the striatum, light delivery activated an inwardly rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light-delivery parameters is required, as even modest introcranial heating can confound interpretation of optogenetic experiments.