Staphylococcus aureus from atopic dermatitis skin alters cytokine production triggered by monocyte-derived Langerhans cell
Staphylococcus aureus from atopic dermatitis skin alters cytokine production triggered by monocyte-derived Langerhans cell
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DOI：
10.1016/j.jdermsci.2017.08.001
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发表时间：
2017-12-01
影响因子：
4.6
通讯作者：
Hide, Michihiro
中科院分区：
文献类型：
--
作者：
Iwamoto, Kazumasa;Moriwaki, Masaya;Hide, Michihiro
背景：特应性皮炎（AD）是最常见的慢性炎症性皮肤病之一。AD患者的皮肤表现为微生物组的失衡，具有强烈的金黄色葡萄球菌定植，这与疾病的严重程度呈正相关。然而，定殖的S.目的：探讨金黄色葡萄球菌对皮肤免疫系统的影响。与标准的金黄色葡萄球菌菌株相比，从AD皮肤分离的金黄色葡萄球菌菌株能够通过朗格汉斯细胞（LC）使T细胞应答偏斜。金黄色葡萄球菌和epidermidis.Methods:我们从健康对照和AD患者中制备单核细胞衍生的LC（MoLC），并用标准的S. aureus NCTC 8325、S.从AD皮肤分离的金黄色葡萄球菌TF 3378或S.表皮将刺激后的MoLC与自体CD 4（POS）T细胞共培养，通过T细胞极化试验、细胞因子分析和实时荧光定量PCR分析T细胞反应。与S. aureus相比，TF 3378诱导的T细胞增殖显著高且快速。aureus NCTC 8325和S.表皮用S.金黄色葡萄球菌TF 3378刺激的MoLC显示出显著高量的IL-2和较少的IFN-γ产生，具有不平衡的Th 1/Th 2（降低的TBX 21/GATA 3比率）mRNA表达。通过S. aureus TF 3378刺激的MoLC可被蛋白酶K抑制。金黄色葡萄球菌TF 3378对AD皮肤的作用可通过LC使T细胞应答偏向不平衡的Th 1/Th 2皮肤免疫。(C)2017日本皮肤病研究学会。由Elsevier爱尔兰有限公司出版。保留所有权利。
Background: Atopic dermatitis (AD) is one of the most common chronic inflammatory skin diseases. The skin of patients with AD presents as a disbalance of the microbiome with a strong colonization by Staphylococcus aureus, which positively correlates with the severity of the disease. However, the effect of colonized S. aureus on the skin immune system has not been fully elucidated.Objective: The aim of this study is to explore whether S. aureus isolated from AD skin is able to skew T cell responses via Langerhans cells (LC) as compared to a standard strain of S. aureus and S. epidermidis.Methods: We prepared monocyte-derived LC (MoLC) from healthy controls and patients with AD, and stimulated MoLC with a standard strain of S. aureus NCTC8325, S. aureus TF3378 isolated from AD skin, or S. epidermidis. Stimulated MoLC were co-cultured with autologous CD4(POS)T cells and then T cell responses were analyzed by T cell polarization assays, cytokine analysis and real-time PCR.Results: MoLC stimulated by S. aureus TF3378 induced significantly high and rapid proliferation of T cells as compared to those by S. aureus NCTC8325 and S. epidermidis. Cytokine productions from T cells cultured with S. aureus TF3378-stimulated MoLC showed significantly high amounts of IL-2 and less IFN-gamma production with imbalanced Th1/Th2 (decreased TBX21/GATA3 ratio) mRNA expression. The T cell proliferation with increased IL-2 production via S. aureus TF3378-stimulated MoLC was diminished by treatment of proteinase K.Conclusion: S. aureus TF3378 on AD skin can skew T cell responses via LC toward imbalanced Th1/Th2 skin immunity. (C) 2017 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.