Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine
Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine
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复合多糖对鸡新城疫及禽流感疫苗的佐剂作用
DOI：
10.1016/j.ijbiomac.2012.01.002
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发表时间：
2012-04-01
影响因子：
8.2
通讯作者：
Gao, Huan
中科院分区：
文献类型：
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作者：
Guo, Liwei;Wang, Deyun;Gao, Huan
本研究通过鸡模型研究复方多糖（cPS）对免疫应答的影响。首先，在筛选实验中，综合分析了黄芪多糖（CAPS）、淫羊藿多糖（EPS）、硫酸APS （sAPS）和硫酸EPS (sEPS) 4种多糖的体外和体内免疫调节活性。筛选出APS-sEPS对淋巴细胞增殖和提高抗体滴度效果最好。因此，进一步验证了APS-sEPS对新城疫（ND）和禽流感（AI）疫苗的佐剂作用。以油佐剂疫苗为对照，分别接种含有150、100和50 mg/kg APS-sEPS的ND或AI疫苗。结果表明，100 mg/kg APS-sEPS可显著提高小鼠ND或AI抗体滴度，增强淋巴细胞增殖。综上所述，适当剂量的APS-sEPS可能是一种安全有效的疫苗免疫刺激物。(C) 2012 Elsevier B.V.版权所有
This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides CAPS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50 mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100 mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines. (C) 2012 Elsevier B.V. All rights reserved.