Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis
Antagonistic interaction between abscisic acid and jasmonate-ethylene signaling pathways modulates defense gene expression and disease resistance in Arabidopsis
复制标题
DOI：
10.1105/tpc.104.025833
复制
发表时间：
2004-12-01
期刊：
影响因子：
11.6
通讯作者：
Kazan, K
中科院分区：
文献类型：
--
作者：
Anderson, JP;Badruzsaufari, E;Kazan, K
植物激素脱落酸（阿坝）、茉莉酸（JA）和乙烯参与多种植物过程，包括在对非生物和生物胁迫的适应性反应期间调节基因表达。以前，阿坝已被牵连在增强疾病的易感性，在各种植物物种，但目前很少有人知道这种现象背后的分子机制。在这项研究中，我们获得的证据表明，阿坝和JA-乙烯信号通路之间的复杂的相互作用调节植物防御基因的表达和抗病性。首先，我们发现外源阿坝抑制防御基因的基础和JA-乙烯激活的转录。相比之下，阿坝缺乏的条件下的突变的ABA 1和ABA 2基因，编码酶参与阿坝的生物合成，导致上调的基础和诱导转录从JA-乙烯响应防御基因。其次，我们发现AtMYC 2（JASMONATE INSENSITIVE 1 [JIN 1]的等位基因），编码一个碱性螺旋-环-螺旋Leu拉链转录因子，这是阿坝信号传导的正调控因子，其破坏导致JA-乙烯应答防御基因的基础和活化转录水平升高。此外，jin 1/myc 2和aba 2 -1突变体表现出对坏死型真菌病原体尖孢镰刀菌的抗性增加。最后，利用乙烯和阿坝信号突变体，我们表明阿坝和乙烯信号之间的相互作用是相互拮抗的营养组织。总的来说，我们的研究结果表明，阿坝和JA-乙烯信号通路的多个组件之间的拮抗作用调节防御和应激反应基因的表达，以响应生物和非生物胁迫。
The plant hormones abscisic acid (ABA), jasmonic acid (JA), and ethylene are involved in diverse plant processes, including the regulation of gene expression during adaptive responses to abiotic and biotic stresses. Previously, ABA has been implicated in enhancing disease susceptibility in various plant species, but currently very little is known about the molecular mechanisms underlying this phenomenon. In this study, we obtained evidence that a complex interplay between ABA and JA-ethylene signaling pathways regulate plant defense gene expression and disease resistance. First, we showed that exogenous ABA suppressed both basal and JA-ethylene-activated transcription from defense genes. By contrast, ABA deficiency as conditioned by the mutations in the ABA1 and ABA2 genes, which encode enzymes involved in ABA biosynthesis, resulted in upregulation of basal and induced transcription from JA-ethylene responsive defense genes. Second, we found that disruption of AtMYC2 (allelic to JASMONATE INSENSITIVE1 [JIN1]), encoding a basic helix-loop-helix Leu zipper transcription factor, which is a positive regulator of ABA signaling, results in elevated levels of basal and activated transcription from JA-ethylene responsive defense genes. Furthermore, the jin1/myc2 and aba2-1 mutants showed increased resistance to the necrotrophic fungal pathogen Fusarium oxysporum. Finally, using ethylene and ABA signaling mutants, we showed that interaction between ABA and ethylene signaling is mutually antagonistic in vegetative tissues. Collectively, our results indicate that the antagonistic interactions between multiple components of ABA and the JA-ethylene signaling pathways modulate defense and stress responsive gene expression in response to biotic and abiotic stresses.