In-Source Decay and Fragmentation Characteristics of Peptides Using 5-Aminosalicylic Acid as a Matrix in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
In-Source Decay and Fragmentation Characteristics of Peptides Using 5-Aminosalicylic Acid as a Matrix in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
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DOI：
10.1016/j.jasms.2010.01.018
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发表时间：
2010-06-01
影响因子：
3.2
通讯作者：
Takayama, Mitsuo
中科院分区：
文献类型：
--
作者：
Sakakura, Motoshi;Takayama, Mitsuo
描述了在基质辅助激光解吸/电离（MALDI）质谱（MS）中使用5-氨基水杨酸（5-阿萨）作为肽（包括单磷酸化和二磷酸化肽）的源内衰变（ISD）的新基质。5-阿萨在MALDI-ISD中的使用已经从几个方面进行了评估：供氢能力，分子和碎片离子峰的突出锐度，以及干扰峰的存在，如亚稳峰和多电荷离子。几种基质如α-氰基-4-羟基肉桂酸（CHCA）、2，5-二羟基苯甲酸（2，5-DHB）、1，5-二氨基萘（1，5-DAN）、芥子酸（SA）、通过使用还原产物[M +2 H + H]（+）与非还原质子化分子[M + H]（+）的峰丰度评价5-阿萨含有二硫键（S S）的环肽加压素。供氢能力大小顺序为1，5-DAN > 5-阿萨> 2，5-DHB > SA = CHCA。化学品1，5-DAN和5-阿萨尤其可被分类为还原性基质。5-阿萨给出了质子化分子和碎片离子比其他基质具有更高锐度的峰，并且在所用肽的ISD质谱中没有给出任何干扰峰，例如多重质子化离子和亚稳离子。特别地，1，5-DAN和5-阿萨给出非常少的亚稳峰。这表明1，5-DAN和5-阿萨比其他基质更“酷”。因此，1，5-DAN和5-阿萨可以被称为“还原冷”基质。此外，它被证实，ISD现象，如N-C α键断裂和还原的S-S键是一个单一的事件在离子源。描述了在MALDI-ISD中形成a-和（a + 2）-系列离子[M + H - 15]（+）、[M + H - 28]（+）和[M + H - 44]（+）离子的特征碎片。（J Am Soc Mass Spectrom 2010，21，979-988）（C）2010年美国质谱学会
The use of 5-aminosalicylic acid (5-ASA) as a new matrix for in-source decay (ISD) of peptides including mono- and di-phosphorylated peptides in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is described. The use of 5-ASA in MALDI-ISD has been evaluated from several standpoints: hydrogen-donating ability, the outstanding sharpness of molecular and fragment ion peaks, and the presence of interference peaks such as metastable peaks and multiply charged ions. The hydrogen-donating ability of several matrices such as alpha-cyarto-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (2,5-DHB), 1,5-diaminonaphthalene (1,5-DAN), sinapinic acid (SA), and 5-ASA was evaluated by using the peak abundance of a reduction product [M + 2H + H](+) to that of non-reduced protonated molecule [M + H](+) of the cyclic peptide vasopressin which contains a disulfide bond (S S). The order of hydrogendonating ability was 1,5-DAN > 5-ASA > 2,5-DHB > SA = CHCA. The chemicals 1,5-DAN and 5-ASA in particular can be classified as reductive matrices. 5-ASA gave peaks with higher sharpness for protonated molecules and fragment ions than other matrices and did not give any interference peaks such as multiply-protonated ions and metastable ions in the ISD mass spectra of the peptides used. Particularly, 1,5-DAN and 5-ASA gave very little metastable peaks. This indicates that 1,5-DAN and 5-ASA are more "cool" than other matrices. The 1,5-DAN and 5-ASA can therefore be termed "reductive cool" matrix. Further, it was confirmed that ISD phenomena such as N-C alpha bond cleavage and reduction of S S bond is a single event in the ion source. The characteristic fragmentations, which form a- and (a + 2)-series ions, [M + H - 15](+), [M + H - 28](+), and [M + H - 44](+) ions in the MALDI-ISD are described. (J Am Soc Mass Spectrom 2010, 21, 979-988) (C) 2010 American Society for Mass Spectrometry