Differentially expressed genes in endothelial differentiation.
Differentially expressed genes in endothelial differentiation.
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DOI：
10.1089/dna.2005.24.432
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发表时间：
2005-07
影响因子：
3.1
通讯作者：
H. Ishii;K. Mimori;M. Mori;A. Vecchione
中科院分区：
文献类型：
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作者：
H. Ishii;K. Mimori;M. Mori;A. Vecchione
通过消减杂交筛选小鼠胚胎干细胞中差异表达的基因，我们确定了三个保守的，但未被鉴定的基因编码bromodomain含有3（BRD 3），蛋白赖氨酸甲基转移酶（PLM），和kelch域含有2（KLHDC 2），这是在内皮细胞分化过程中下调。RNA印迹研究显示，这些基因在未分化的ES细胞中显著表达，而在内皮分化后表达降低;小鼠内皮的研究显示BRD 3的表达显著降低。一项对位于9号染色体q34处的人类BRD 3（一个易受基因组重排影响的区域）的研究显示，与邻近的非癌组织相比，12名膀胱癌患者中有4名的表达发生了改变。结合显示BRD 3对细胞增殖的正调节的siRNA抑制的结果，表明该分子在允许细胞进入血管生成过程的增殖期中起作用。
By screening differentially expressed genes in mouse embryonic stem (ES) cells by subtractive hybridization, we identified three conserved but uncharacterized genes encoding bromodomain containing 3 (BRD3), protein lysine methyltransferase (PLM), and kelch domain containing 2 (KLHDC2), which were downregulated during endothelial differentiation. An RNA blot study showed that these genes were markedly expressed in undifferentiated ES cells, whereas the expression was reduced upon endothelial differentiation; a study of mouse endothelium showed a significant reduction in the expression of BRD3. A study of human BRD3, located on chromosome 9 at q34, a region susceptible to genomic rearrangement, showed an altered expression in 4 of 12 patients with bladder cancer, compared with adjacent noncancerous tissues. Taken together with the result of siRNA inhibition showing the positive regulation of cell proliferation by BRD3, it is suggested that this molecule plays a role in allowing cells to enter the proliferative phase of the angiogenic process.