ASSEMBLED BACULOVIRUS-EXPRESSED HUMAN PAPILLOMAVIRUS TYPE-11 L1 CAPSID PROTEIN VIRUS-LIKE PARTICLES ARE RECOGNIZED BY NEUTRALIZING MONOCLONAL-ANTIBODIES AND INDUCE HIGH TITERS OF NEUTRALIZING ANTIBODIES
ASSEMBLED BACULOVIRUS-EXPRESSED HUMAN PAPILLOMAVIRUS TYPE-11 L1 CAPSID PROTEIN VIRUS-LIKE PARTICLES ARE RECOGNIZED BY NEUTRALIZING MONOCLONAL-ANTIBODIES AND INDUCE HIGH TITERS OF NEUTRALIZING ANTIBODIES
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DOI：
10.1099/0022-1317-75-9-2271
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发表时间：
1994-09-01
影响因子：
3.8
通讯作者：
KREIDER, JW
中科院分区：
文献类型：
--
作者：
CHRISTENSEN, ND;HOPFL, R;KREIDER, JW
在昆虫细胞中产生杆状病毒表达的人乳头瘤病毒11型（HPV-11）主要衣壳蛋白（L1）病毒样颗粒（VLP），并在CsCl密度梯度上纯化。VLP保留了通过一系列HPV-11中和单克隆抗体检测到的构象中和表位。电子显微镜检查确定HPV-11 L1 VLP大小可变，表面形貌与感染性HPV-11相似。VLP是非常抗原性的，并且当用作免疫原而没有商业佐剂制剂时，在兔和小鼠中诱导高滴度的中和抗体。这些VLP试剂可能是预防HPV感染的有效疫苗。
Baculovirus-expressed human papillomavirus type 11 (HPV-11) major capsid protein (L1) virus-like particles (VLPs) were produced in insect cells and purified on CsCl density gradients. The VLPs retained conformational neutralizing epitopes that were detected by a series of HPV-11-neutralizing monoclonal antibodies. Electron microscopy determined that the HPV-11 L1 VLPs were variable in size with a surface topography similar to that of infectious HPV-11. The VLPs were very antigenic, and induced high titres of neutralizing antibodies in rabbits and mice when used as an immunogen without commercial preparations of adjuvant. These VLP reagents may be effective vaccines for protection against HPV infections.