Alcohol metabolizing enzymes: Studies of isozymes in human biopsies and cultured fibroblasts
Alcohol metabolizing enzymes: Studies of isozymes in human biopsies and cultured fibroblasts
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酒精代谢酶：人体活检和培养成纤维细胞中同工酶的研究
DOI：
10.1111/j.1399-0004.1979.tb00845.x
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发表时间：
1979
影响因子：
3.5
通讯作者：
S. Harada
中科院分区：
文献类型：
--
作者：
H. Goedde;D. Agarwal;S. Harada
本文介绍了皮肤提取物、培养的成纤维细胞和其他器官中乙醇脱氢酶和乙醛脱氢酶同工酶的快速、灵敏的显微研究方法。讨论了将这些技术应用于研究对酒精反应的个体间差异的可能性。一名日本人的皮肤活检组织培养的成纤维细胞显示出酒精脱氢酶的异二聚体(ADH22-1)，而另一名日本人的皮肤提取液显示出同源二聚体(ADH22-2)。在人体不同器官中检测到多达四组乙醛脱氢酶(ALDH)同工酶，在皮肤和成纤维细胞提取物中至少发现三组。我们对肝脏、胃和皮肤的初步数据表明，ALDH是多态的，这些同工酶组的确定与几个基因座有关。
Rapid and sensitive micromethods for the study of alcohol dehydrogenase and aldehyde dehydrogenase isozymes in skin extracts, cultured fibroblasts and other organs are presented. Possibilities for the application of these techniques to the study of interindividual variations in response to alcohol are discussed. While fibroblasts cultured from a skin biopsy from one Japanese individual revealed a heterodimer (ADH22‐1) of alcohol dehydrogenase, skin extract from another Japanese showed a homodimer (ADH22‐2). Up to four isozyme sets for aldehyde dehydrogenase (ALDH) were detected in various human organs and at least three sets were found in skin and fibroblast extracts. Our preliminary data on liver, stomach, and skin indicate that ALDH is polymorphic and several loci are concerned in the determination of these isozyme sets.