Translational Research and Innovation in Human and Health Science
Translational Research and Innovation in Human and Health Science
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人类与健康科学的转化研究与创新
DOI：
10.1080/07853890.2018.1427452
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发表时间：
2018
影响因子：
4.4
通讯作者：
Gérard Keith
中科院分区：
文献类型：
--
作者：
G. Pixa;Guy Dirheimer;Gérard Keith
红细胞聚集是心血管风险的指标，其受血浆纤维蛋白原浓度的影响。纤维蛋白原水平在心血管疾病期间升高。我们的主要目标是了解纤维蛋白原-红细胞结合如何影响红细胞聚集，以及它如何构成原发性动脉高血压（EAH）和慢性心力衰竭（CHF）的心血管危险因素。纤维蛋白原-红细胞和红细胞-红细胞粘附测量进行了原子力显微镜（AFM）为基础的力谱。随着纤维蛋白原浓度的增加，无论是健康供体还是EAH患者，细胞-细胞分离所需的功和力都增加。然而，在每个纤维蛋白原浓度下，EAH患者获得了更高的值。与对照组相比，EAH和CHF患者的纤维蛋白原-红细胞结合力较高，尽管结合频率较低。与非缺血性患者相比，缺血性CHF患者显示结合力增加。红细胞变形性（评估为伸长指数）结果显示，心力衰竭患者在较低剪切应力下呈现出比对照组更高的红细胞变形性，并且在较高剪切应力下呈现出更低的变形性。这表明，在内径较大的血管中，患者的红细胞比健康供体的红细胞更易变形;然而，在直径较小的血管中，存在相反的趋势。最后，12个月的临床随访显示，在评估开始时通过AFM探测的纤维蛋白原-红细胞结合力较高的CHF患者，在随后的一年中由于心血管并发症而住院的概率显著较高。我们的研究结果表明，原子力显微镜可以成为一个有前途的工具，临床预后，精确定位那些患者的心血管疾病的风险增加。
Erythrocyte aggregation is an indicator of cardiovascular risk, which is influenced by plasma fibrinogen concentration. Fibrinogen levels are elevated during cardiovascular diseases. Our main goals were to understand how fibrinogen-erythrocyte binding influences erythrocyte aggregation and how it constitutes a cardiovascular risk factor in essential arterial hypertension (EAH) and chronic heart failure (CHF). Fibrinogen-erythrocyte and erythrocyte-erythrocyte adhesion measurements were conducted by atomic force microscopy (AFM)-based force spectroscopy. Upon increasing fibrinogen concentration, there was an increase in the work and force necessary for cell-cell detachment, both for healthy donors and EAH patients. Nevertheless, higher values were obtained for the EAH patients at each fibrinogen concentration. Fibrinogen-erythrocyte (un) binding forces were higher in EAH and CHF patients, when compared with the control group, despite a lower binding frequency. Ischemic CHF patients showed increased binding forces compared to non-ischemic patients. Erythrocyte deformability (assessed as elongation index) results show that heart failure patients presented higher erythrocyte deformability than the control group at lower shear stresses, and lower deformability at higher shear stresses. This indicates that patients’ erythrocytes are more deformable than those from healthy donors in blood vessels with larger internal diameters; however, in smaller-diameter vessels the opposite trend exists. Finally, a 12-month clinical follow-up shows that CHF patients with higher fibrinogen-erythrocyte binding forces, probed by AFM at the beginning of the assessment, had a significantly higher probability of being hospitalized due to cardiovascular complications on the subsequent year. Our results show that AFM can be a promising tool for clinical prognosis, pinpointing those patients with increased risk for cardiovascular diseases.