Calpain 3 is expressed in astrocytes of rat and Microcebus brain
Calpain 3 is expressed in astrocytes of rat and Microcebus brain
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DOI：
10.1016/s0891-0618(02)00102-3
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发表时间：
2003-02-01
影响因子：
2.8
通讯作者：
Benyamin, Y
中科院分区：
文献类型：
--
作者：
König, N;Raynaud, F;Benyamin, Y
钙依赖性蛋白酶calpain参与许多功能，包括控制细胞存活、可塑性和运动性。尽管同种异构体calpain1和calpain2被描述为普遍表达的酶，但calpain3被称为“肌肉特异性”酶，尽管通过Northern blot在脑匀浆中检测到微量的calpain3 mRNA。在这项研究中，我们验证了针对特定于给定同种异构体的肽或存在于所有三种同种异构体中的肽的抗体。然后，我们将抗calpain 3抗体与针对细胞类型特异性蛋白的抗体一起使用，通过双标记和三标记免疫细胞化学来确定蛋白酶是否在大鼠和小鼠狐猴（Microcebus murinus）大脑的特定细胞群中表达。calpain3几乎只存在于星形胶质细胞形态的细胞中。这些细胞，其中大多数共表达胶质纤维酸性蛋白，在纹状体脑室下区（大量神经元形成向嗅球的吻侧迁移流（RMS））和RMS本身附近特别多。其他免疫反应细胞在靠近前脑枕表面、胼胝体和齿状回中被发现。钙蛋白酶3可能参与星形胶质细胞的可塑性和/或运动性。(C) 2002 Elsevier Science B.V.版权所有
The calcium-dependent protease calpain is involved in numerous functions, including the control of cell survival, plasticity and motility. Whereas the isoforms calpain 1 and 2 have been described as ubiquitously expressed enzymes, calpain 3 has been called, "muscle-specific", although trace amounts of calpain 3 mRNA have been detected by Northern blot in brain homogenates. In this study, we validated antibodies raised either against the peptides that were specific for a given isoform or the peptides present in all the three isoforms. We then used the anti-calpain 3 antibodies together with antibodies directed against cell-type-specific proteins to determine by double- and triple-labelling immunocytochemistry if the protease is expressed in specific cell populations of rat as well as lesser mouse lemur (Microcebus murinus) brain. Calpain 3 was almost exclusively found in cells displaying astrocyte morphology. These cells, most of which co-expressed glial fibrillary acidic protein, were particularly numerous close to the striatal subventricular zone (where numerous neurones forming the rostral migratory stream (RMS) towards the olfactory bulbs are generated) and the RMS itself. Other immunoreactive cells were found close to the pial surface of the forebrain, in the corpus callosum and in the dentate gyrus. Calpain 3 may be involved in astrocyte plasticity and/or motility. (C) 2002 Elsevier Science B.V. All rights reserved.