Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia
Alterations of serum cytokine levels and their relation with inflammatory markers in candidemia
复制标题
DOI：
10.1093/mmy/myu084
复制
发表时间：
2015-04-01
期刊：
影响因子：
2.9
通讯作者：
Oral, Haluk Barbaros
中科院分区：
文献类型：
--
作者：
Akin, Hicran;Akalin, Halis;Oral, Haluk Barbaros
CRP、PCT、血清淀粉样蛋白A（SAA）和细胞因子在真菌感染诊断中的作用尚未明确。本研究旨在检测念珠菌血症患者血清中白细胞介素（IL）-23、IL-17、IL-1 β、肿瘤坏死因子（TNF）-α、IL-10、转化生长因子（TGF）-β、C-反应蛋白（CRP）、降钙素原（PCT）和血清淀粉样蛋白A（SAA）的水平，并与菌血症患者进行比较。将50名念珠菌血症患者的血清细胞因子水平与14名多微生物败血症患者、30名菌血症患者和27名健康对照者的血清细胞因子水平进行比较。根据生产商的方案，使用夹心ELISA研究细胞因子水平，发现与菌血症患者和健康对照组的样本相比，念珠菌血症组的TGF-β、IL-23和IL-17的血清水平显著较高。菌血症组的PCT和SAA水平高于念珠菌血症组和健康对照组。假设IL-17水平阈值> 38.79 pg/ml，则敏感性和特异性分别为38%和96.6%，而假设IL-23水平阈值> 59.97 pg/ml，则敏感性和特异性分别为72%和60%。当TGF-β阈值> 560 pg/ml时，TGF-β水平的敏感性和特异性分别为85.71%和53.33%。PCT和SAA在念珠菌血症和菌血症的鉴别诊断中表现出上级的性能，我们的研究表明IL-17、IL-23、TGF-β、PCT和SAA水平可以作为念珠菌血症的诊断标志物
The roles of CRP, PCT, serum amyloid A (SAA), and cytokines in the diagnosis of fungal infections have not yet been clearly demonstrated. This study aims to measure the serum levels of interleukin (IL)-23, IL-17, IL-1 beta, tumor necrosis factor (TNF)-alpha, IL-10, transforming growth factor (TGF)-beta, C-reactive protein (CRP), procalcitonin (PCT), and serum amyloid A (SAA) in cases of candidemia and to compare them with those observed in cases of bacteremia.For this purpose, the serum cytokine levels from 50 patients with candidemia were compared with those of 14 patients with polymicrobial sepsis, 30 patients with bacteremia, and 27 healthy control subjects. The cytokine levels were studied using sandwich ELISAs according to the manufacturer protocol.The serum levels of TGF-beta, IL-23, and IL-17 were found to be significantly higher in the candidemia group in comparison with the samples from those with bacteremia and healthy controls. The PCT and SAA levels were higher in samples from the group with bacteremia those from individuals with candidemia and the healthy control group. Assuming an IL-17 level threshold of > 38.79 pg/ml, the sensitivity and specificity were 38% and 96.6%, respectively but considering an IL-23 threshold of > 59.97 pg/ml, the sensitivity and specificity valueswere found to be 72% and 60%, respectively. The sensitivity and the specificity of the TGF-beta levels were found to be 85.71% and 53.33%, respectively, when the TGF-beta threshold is > 560 pg/ml. PCT and SAA demonstrated a superior performance for the differentiation of candidemia and bacteremia.Our study demonstrates that IL-17, IL-23, TGF-beta, PCT, and SAA levels could be a diagnostic marker for candidemia