BENZYLADENINE-INDUCED STIMULATION OF 5-AMINOLEVULINIC ACID ACCUMULATION UNDER VARIOUS LIGHT INTENSITIES IN LEVULINIC ACID-TREATED COTYLEDONS OF ETIOLATED CUCUMBER
BENZYLADENINE-INDUCED STIMULATION OF 5-AMINOLEVULINIC ACID ACCUMULATION UNDER VARIOUS LIGHT INTENSITIES IN LEVULINIC ACID-TREATED COTYLEDONS OF ETIOLATED CUCUMBER
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DOI：
10.1111/j.1399-3054.1985.tb02329.x
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
DEI, M
中科院分区：
文献类型：
--
作者：
DEI, M
在用43μmol m-2 s-1光照射时，在切下的黄瓜(Cucumis sativus L.cv.Aonagajibai)黄化子叶中，在乙酰丙酸存在下，苄基腺嘌呤(BA)刺激5-氨基乙酰丙酸(ALA)积累。用 BA 进行短暂的暗预处理（6 小时）消除了 ALA 积累的滞后期。与用BA预处理6小时的子叶相比，用BA长时间(14小时)预处理的子叶在稳态阶段期间ALA的积累速率显着加快。在 BA 预处理和水控子叶中，滞后期 ALA 积累速率在非常低的光通量（< 1.4 μmol m-2 s-1）下达到饱和。在控水子叶中，ALA积累的稳态速率随着光通量的增加而增加，直至43 μmol m-12 s-1（与叶绿素[Chl]形成的速率平行）。在用 BA 预处理 6 或 14 小时的子叶中，稳态速率在非常低的光通量下达到稳定水平。讨论了受BA和光强度影响的叶绿素生物合成途径可能的中间步骤。
Benzyladenine (BA) stimulated 5-aminolevulinic acid (ALA) accumulation in the presence of levulinic acid during illumination with 43 .mu.mol m-2 s-1 light in excised etiolated cotyledons of cucumber (Cucumis sativus L. cv. Aonagajibai). A short dark-pretreatment (6h) with BA eliminated the lag phase of ALA accumulation. The rate of ALA accumulation during the steady-state phase in cotyledons pretreated with BA for a long period (14 h) was considerably accelerated compared to that in cotyledons pretreated with BA for 6 h. The rate of ALA accumulation during the lag phase was saturated at a very low light fluence (< 1.4 .mu.mol m-2 s-1) in both BA-pretreated and water-control cotyledons. The steady-state rate of ALA accumulation increased with increasing light fluence up to 43 .mu.mol m-12 s-1 (parallel to that of chlorophyll[Chl] formation) in water-control cotyledons. In cotyledons pretreated with BA for either 6 or 14 h, the steady-state rate reached a plateau at a very low light fluence. Possible intermediate steps of Chl biosynthesis pathway affected by BA and light intensity are discussed.