Detection of salt bridges to lysines in solution in barnase
Detection of salt bridges to lysines in solution in barnase
复制标题
DOI：
10.1039/c3cc45602a
复制
发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Hansen, Poul Erik
中科院分区：
文献类型：
--
作者：
Williamson, Mike P.;Hounslow, Andrea M.;Hansen, Poul Erik
我们表明，涉及赖氨酸的盐桥可以检测到氘同位素的NMR化学位移的侧链胺的影响。核糖核酸酶芽孢杆菌RNA酶中的Lys27是盐桥，Arg69突变为Lys保留了部分掩埋的盐桥。盐桥在功能上很重要。
We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.