Characterization of three putative sub-domains in the signal-input domain of the ArcB hybrid sensor in Escherichia coli(1).
Characterization of three putative sub-domains in the signal-input domain of the ArcB hybrid sensor in Escherichia coli(1).
复制标题
大肠杆菌 ArcB 混合传感器信号输入域中三个假定子域的表征 (1)。
DOI：
10.1093/oxfordjournals.jbchem.a022679
复制
发表时间：
2000
影响因子：
2.7
通讯作者：
T. Mizuno
中科院分区：
文献类型：
--
作者：
A. Matsushika;T. Mizuno
ArcB传感器在组氨酸到天冬氨酸(His-to-Asp)磷酸转导中起着至关重要的作用，该信号转导参与了转录调控网络，使大肠杆菌细胞能够感知各种呼吸道生长条件。ArcB是参与多步His-to-Asp磷酸传递过程中研究最多的混合His-Kinase之一。然而，一个有待阐明的主要问题是：ArcB是如何感知缺氧信号的？ArcB的N-末端区域被认为是一个信号输入域，可能在这种信号感知中发挥作用。在这项研究中，ArcB的这个N-末端区域被分成三个假定的亚区，一个是“跨膜区”，一个是“亮氨酸拉链样区”，还有一个是“PAS样区”。通过系统分析一些arcB突变体，在体内和体外评估了这些结构域的重要性，每个突变都编码一个突变的ArcB蛋白，在这些亚域中的一个亚域中有氨基酸替代或缺失。在讨论结果时特别参考了ArcB厌氧传感器的性质。
The ArcB sensor plays a crucial role in the histidine to aspartate (His-to-Asp) phosphorelay signal transduction, which is involved in the transcriptional regulatory network that allows Escherichia coli cells to sense various respiratory growth conditions. ArcB is one of the best-studied hybrid His-kinases involved in the multi-step His-to-Asp phosphorelay. However, a major question that remains to be elucidated is: how does ArcB sense an anoxic signal? The N-terminal region of ArcB is considered to be a signal-input domain, which probably plays a role in such signal-perception. In this study, this N-terminal region of ArcB was dissected into three putative sub-domains, a "transmembrane domain," a "leucine-zipper-like domain, " and a "PAS-like domain." The importance of these structural domains was assessed in vivo and in vitro by systematically analyzing a number of arcB mutants, each of which encodes a mutant ArcB protein having an amino acid substitution or a deletion within one of these sub-domains. The results are discussed with special reference to the nature of the ArcB anaerobic sensor.