Development of nanoparticle libraries for biosensing
Development of nanoparticle libraries for biosensing
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DOI：
10.1021/bc050290e
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发表时间：
2006-01-01
影响因子：
4.7
通讯作者：
Weissleder, R
中科院分区：
文献类型：
--
作者：
Sun, EY;Josephson, L;Weissleder, R
磁性和磁荧光纳米颗粒已成为生物应用的重要材料，特别是用于传感，分离和成像。为了实现目标特异性，这些纳米材料通常用结合蛋白（例如抗体或蛋白质）进行共价修饰。在这里，我们报告了通过小分子的多价修饰实现特异性的纳米颗粒库的创建。我们探索了不同的合成路线，将小分子与酸酐，胺，羟基羧基，硫醇和环氧手柄连接。我们表明，衍生的纳米材料具有独特的生物功能，在细胞筛选中具有不同的行为，可以用作生物筛选的基底。
Magnetic and magnetofluorescent nanoparticles have become important materials for biological applications especially for sensing, separation, and imaging. To achieve target specificity, these nanomaterials are often covalently modified with binding proteins such as antibodies or proteins. Here we report on the creation of nanoparticle libraries that achieve specificity through multivalent modification with small molecules. We explore different synthetic routes to attach small molecules with anhydride, amine, hydroxyl carboxyl, thiol, and epoxy handles. We show that the derived nanomaterials have unique biological functions, possess different behaviors in cell screens, and can be used as substrates for biological screens.