Focal adhesion kinase localizes to sites of cell-to-cell contact in vivo and increases apically in rat uterine luminal epithelium and the blastocyst at the time of implantation
Focal adhesion kinase localizes to sites of cell-to-cell contact in vivo and increases apically in rat uterine luminal epithelium and the blastocyst at the time of implantation
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DOI：
10.1002/jmor.20010
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发表时间：
2012-06-01
影响因子：
1.5
通讯作者：
Murphy, Christopher R.
中科院分区：
文献类型：
--
作者：
Kaneko, Yui;Lecce, Laura;Murphy, Christopher R.
局灶性粘连在促进胚胎入侵方面发挥着重要作用;特别是，局灶性粘连在大鼠着床时解体，促进子宫腔上皮脱落，使胚胎入侵子宫内膜。本研究探讨了粘着斑蛋白，粘着斑激酶（FAK）在大鼠子宫腔，腺上皮细胞，以了解在早孕粘着斑的动力学。FAK在妊娠早期经历了广泛的分布变化，令人惊讶的是，FAK并不局限于粘着斑的部位，而是局限于细胞与细胞接触的部位，并在妊娠第1天与ZO-1共定位。在着床时，子宫腔上皮细胞的顶端区域FAK增加，这是由孕酮调节的。在体外共培养的大鼠胚泡与石川细胞的模型中，FAK在大鼠胚泡和石川细胞的顶端都有表达，提示FAK在这两种遗传上不同的细胞类型之间的粘附和介导信号转导中起作用。J. Morphol.，2012. (C)2012年威利期刊公司
Focal adhesions play an important role in promoting embryo invasion; in particular, focal adhesions disassemble at the time of implantation in the rat, facilitating the detachment of the uterine luminal epithelium to allow the embryo to invade the endometrium. This study investigated focal adhesion protein, focal adhesion kinase (FAK) in the rat uterine luminal, and glandular epithelial cells to understand the dynamics of focal adhesions during early pregnancy. FAK undergoes extensive distributional change during early pregnancy, and surprisingly, FAK was not localized at the site of focal adhesions, instead being localized to the site of cell-to-cell contact and colocalizing with ZO-1 on day 1 of pregnancy. At the time of implantation, FAK increases in the apical region of the uterine luminal epithelial cells which was regulated by progesterone. Using an in vitro co-culture model of rat blastocysts attached to Ishikawa cells, FAK was present apically both in the rat blastocyst and the Ishikawa cells, suggesting a role in attachment andin mediating signal transduction between these two genetically different cell types. J. Morphol., 2012. (C) 2012 Wiley Periodicals, Inc.