Xylella fastidiosa requires polygalacturonase for colonization and pathogenicity in Vitis vinifera grapevines
Xylella fastidiosa requires polygalacturonase for colonization and pathogenicity in Vitis vinifera grapevines
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DOI：
10.1094/mpmi-20-4-0411
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发表时间：
2007-04-01
影响因子：
3.5
通讯作者：
Kirkpatrick, Bruce C.
中科院分区：
文献类型：
--
作者：
Roper, M. Caroline;Greve, L. Carl;Kirkpatrick, Bruce C.
顶端木霉是葡萄皮尔斯氏病的病原，葡萄皮尔斯病是葡萄生产中的一种重大经济病害。该菌系统地侵染葡萄的木质部分子，并能以未知的机制突破分隔木质部导管的孔孔膜。我们推测，该菌利用细胞壁降解酶来分解坑膜，这是基于该菌基因组中存在与植物细胞壁降解有关的基因。这些基因包括几个β-1，4内切葡聚糖酶、几个木聚糖酶、几个木糖苷酶和一个多聚半乳糖醛酸酶(PG)。在这项研究中，我们证明了pglA基因编码一个功能性的PG。PglA中的一个突变体失去了致病性，系统地定植葡萄的能力受到了损害。结果表明，Pg是青霉成功侵染葡萄所必需的，是青霉致病的关键致病因子。
Xylella fastidiosa is the causal agent of Pierce's disease of grape, an economically significant disease for the grape industry. X. fastidiosa systemically colonizes the xylem elements of grapevines and is able to breach the pit pore membranes separating xylem vessels by unknown mechanisms. We hypothesized that X. fastidiosa utilizes cell wall degrading enzymes to break down pit membranes, based on the presence of genes involved in plant cell wall degradation in the X. fastidiosa genome. These genes include several beta-1,4 endoglucanases, several xylanases, several xylosidases, and one polygalacturonase (PG). In this study, we demonstrated that the pglA gene encodes a functional PG. A mutant in pglA lost pathogenicity and was compromised in its ability to systemically colonize Vitis vinifera grapevines. The results indicate that PG is required for X. fastidiosa to successfully infect grapevines and is a critical virulence factor for X. fastidiosa pathogenesis in grapevine.