The expression of KATP channel subunits in alpha-synuclein-transfected MES23.5 cells
The expression of KATP channel subunits in alpha-synuclein-transfected MES23.5 cells
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α-突触核蛋白转染的MES23.5细胞中K-ATP通道亚基的表达
DOI：
10.21037/atm.2018.04.24
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发表时间：
2018-05-01
影响因子：
--
通讯作者：
Jiang, Hong
中科院分区：
文献类型：
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作者：
Han, Shuai-Shuai;Jiao, Qian;Jiang, Hong
背景资料：SUR 1是ATP敏感性钾通道（K-ATP）的亚基之一，在帕金森病（PD）患者的黑质（SN）有高表达。虽然多巴胺（DA）神经元选择性死亡的机制尚不清楚，但已有研究表明，选择性激活黑质K-ATP通道可能与DA神经元的变性有关。本研究的目的是用α-突触核蛋白检测多巴胺能细胞K-ATP通道亚单位的表达方法：采用实时荧光定量PCR技术检测α-Syn转染后的MES23.5细胞K-ATP通道亚基mRNA水平。我们的结果显示，SUR 1亚基的mRNA水平在WT α-Syn过表达细胞中显著增加了35%，在A53 T α-Syn过表达细胞中显著增加了31%。结论：我们的研究表明，在α-Syn过表达的MES23.5细胞中，SUR 1的mRNA水平选择性上调，而SUR 2B和Kir6.2的mRNA水平则无明显变化。研究结果表明，SUR 1的过度表达可能参与了PD的发生发展过程。
Background: SUR1, one of the subunits of ATP-sensitive potassium (K-ATP) channels, was found to be highly expressed in mRNA levels in the substantia nigra (SN) of Parkinson's disease (PD) brains. Though the mechanism of the selective dopamine (DA) neurons death is still unknown, some studies have demonstrated that selective activation of the K-ATP channels in the SN might be associated with the degeneration of DA neurons. The objective of our study is to examine the expressions of K-ATP channel subunits in dopaminergic cells with alpha-synuclein (alpha-Syn) transfection.Methods: In this study, we detected the K-ATP channel subunits mRNA levels in MES23.5 cells by real-time quantitative PCR after the cells transfected with alpha-Syn.Results: Our results showed that the mRNA levels of SUR1 subunit were markedly increased by 35% in WT alpha-Syn overexpression cells and by 31% in A53T alpha-Syn overexpression cells, respectively. However, the mRNA levels of SUR2B and Kir6.2 subunit have no obviously differences from the controls.Conclusions: We showed that the mRNA levels of SUR1 but not SUR2B or Kir6.2 were selectively upregulated in MES23.5 cells over-expressed with alpha-Syn. The findings demonstrated that the SUR1 overexpressed might be involved in the process of PD.