Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses
Optical reprogramming of human somatic cells using ultrashort Bessel-shaped near-infrared femtosecond laser pulses
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DOI：
10.1117/1.jbo.20.11.115008
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发表时间：
2015-11
影响因子：
3.5
通讯作者：
A. Uchugonova;H. Breunig;A. Batista;K. König
中科院分区：
文献类型：
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作者：
A. Uchugonova;H. Breunig;A. Batista;K. König
抽象的。我们报告了一种无病毒的光学方法，使用超短贝塞尔形状的激光脉冲，通过低功率纳米孔将人类细胞重编程为诱导多能干细胞。采用高重复频率85 MHz的皮焦耳近红外亚20 fs激光脉冲在真皮成纤维细胞膜中产生瞬时纳米孔，用于引入四种转录因子以诱导重编程过程。与利用逆转录病毒或慢病毒将基因或转录因子递送到宿主基因组中的常规方法相比，激光方法是无病毒的;因此，避免了限制临床应用的病毒诱导的癌症产生的风险。
Abstract. We report a virus-free optical approach to human cell reprogramming into induced pluripotent stem cells with low-power nanoporation using ultrashort Bessel-shaped laser pulses. Picojoule near-infrared sub-20 fs laser pulses at a high 85 MHz repetition frequency are employed to generate transient nanopores in the membrane of dermal fibroblasts for the introduction of four transcription factors to induce the reprogramming process. In contrast to conventional approaches which utilize retro- or lentiviruses to deliver genes or transcription factors into the host genome, the laser method is virus-free; hence, the risk of virus-induced cancer generation limiting clinical application is avoided.