Src-family kinase-p53/ Lyn p56 plays an important role in TNF-alpha-stimulated production of O2- by human neutrophils adherent to fibrinogen.
Src-family kinase-p53/ Lyn p56 plays an important role in TNF-alpha-stimulated production of O2- by human neutrophils adherent to fibrinogen.
复制标题
Src 家族激酶 -p53/Lyn p56 在 TNF-α 刺激粘附纤维蛋白原的人中性粒细胞产生 O2- 中发挥重要作用。
DOI：
10.1023/a:1020245129632
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发表时间：
1999
期刊：
影响因子：
5.1
通讯作者：
Novak,MJ
中科院分区：
文献类型：
--
作者：
Yan,SR;Novak,MJ
Stimulation of neutrophil function by TNFα is largely dependent on β2integrins. It has also been proposed thatsrc-family kinases are involved in this process. However, the functions ofsrc-like kinases in human neutrophils still remain to be determined. In the present study, we used the newsrc-family kinase specific inhibitor PP1 4-Amino-5-(4-methylphenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine to investigate the rolesrc-kinases play in TNFα stimulation of neutrophil function. Our results demonstrated that, in neutrophils adherent to fibrinogen, PP1 inhibited TNFα-stimulated superoxide production and protein tyrosine phosphorylation in a dose-dependent manner. In in vitro kinase assays, PP1 profoundly inhibited the activation of p53/56lynbut not p59hckor p72syk. Only slight inhibition was found of p58c-fgr. These data indicate that p53/56lynplays an important role in TNFα-mediated stimulation of PMN function.
