EDR1 associates with its homologs to synergistically regulate plant immunity in Arabidopsis.
EDR1 associates with its homologs to synergistically regulate plant immunity in Arabidopsis.
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DOI：
10.1016/j.plantsci.2023.111619
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发表时间：
2023-02
期刊：
影响因子：
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通讯作者：
Guitao Zhong;Yongming Chen;Simu Liu;Chenyang Gao;Renjie Chen;Zhanchun Wang;Wei Wang;Dingzhong Tang
中科院分区：
文献类型：
--
作者：
Guitao Zhong;Yongming Chen;Simu Liu;Chenyang Gao;Renjie Chen;Zhanchun Wang;Wei Wang;Dingzhong Tang
ENHANCED DISEASE RESISTANCE 1 (EDR1), a Raf-like mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK), is a negative regulator of resistance. There are three homologs, RAF3/4/5, of EDR1 in Arabidopsis. However, the roles of RAF3/4/5 in resistance and their functional link with EDR1 in plant immunity remain unclear. Here, we showed that theraf3/4/5triple mutant displayed wild-type-like phenotypes to the powdery mildew pathogenGolovinomycescichoracearumUCSC1 and the bacterial pathogen Pseudomonas syringae pv.tomato(Pto) DC3000. However, theedr1 raf3/4/5quadruple mutant exhibited enhanced resistance toG. cichoracearumUCSC1 andPtoDC3000 compared toedr1. Consistently, MPK3/6 kinase activity was more highly activated inedr1 raf3/4/5than that inedr1. Moreover, the enhanced resistance ofedr1 raf3/4/5required SALICYLIC ACID INDUCTION DEFICIENT 2 (SID2), an isochorismate synthase required for salicylic acid (SA) synthesis.Additionally, unlike EDR1, RAF3/4/5 weakly and indirectly associated with MKK4/5, and EDR1 was directly associated with RAF3/4/5. Taken together, these data indicate that EDR1 associates with RAF3/4/5, and they may function together to synergistically suppress MAPK cascades activation, which reveal the complexity and importance of Raf-like MAPKKKs in plant immunity regulation.
