Replication fork collapse at replication terminator sequences
Replication fork collapse at replication terminator sequences
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DOI：
10.1093/emboj/cdf369
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发表时间：
2002-07-15
期刊：
影响因子：
11.4
通讯作者：
Michel, B
中科院分区：
文献类型：
--
作者：
Bidnenko, V;Ehrlich, SD;Michel, B
复制叉停滞是基因组重排的一个来源，并且被阻断的复制叉的重组发生特性可能取决于阻断的原因。在这里，我们研究的命运复制叉在自然复制逮捕网站。为此，将大肠杆菌复制终止子序列Ter置于细菌染色体上的异位位置。所得到的菌株需要重组修复才能存活，但在Ter阻断的复制叉不会被破坏。线性DNA分子在第二轮复制叉到达时形成，第二轮复制叉将第一个被阻断的叉的DNA链复制到末端。提出了一个模型，占的同源重组的活力，尽管缺乏染色体断裂的要求。这项工作表明，自然和意外复制逮捕网站的处理不同。
Replication fork arrest is a source of genome re arrangements, and the recombinogenic properties of blocked forks are likely to depend on the cause of blockage. Here we study the fate of replication forks blocked at natural replication arrest sites. For this purpose, Escherichia coli replication terminator sequences Ter were placed at ectopic positions on the bacterial chromosome. The resulting strain requires recombinational repair for viability, but replication forks blocked at Ter are not broken. Linear DNA molecules are formed upon arrival of a second round of replication forks that copy the DNA strands of the first blocked forks to the end. A model that accounts for the requirement for homologous recombination for viability in spite of the lack of chromosome breakage is proposed. This work shows that natural and accidental replication arrests sites are processed differently.