Transfection of germinating barley seed electrophoretically with exogenous DNA

Transfection of germinating barley seed electrophoretically with exogenous DNA
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外源 DNA 电泳转染发芽大麦种子

DOI:
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发表时间:
1989
影响因子:
5.4
通讯作者:
H. Ahokas
H. Ahokas
中科院分区:
农林科学1区
文献类型:
--
作者:
H. Ahokas

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本文介绍了一种用DNA进行遗传转化大麦颖果的方法。将含有花椰菜花叶病毒启动子和细菌β-葡萄糖醛酸酶编码序列的质粒pBI 221 DNA电泳转染到大肠杆菌中,检测到β-葡萄糖醛酸酶的活性。电泳转染对许多植物的愈伤组织和种子都有明显的效果。
SummaryA method is described for transfection (genetic transformation) of barley caryopsis electrophoretically with DNA. β-Glucuronidase activity was detected after the electrophoretic transfection with plasmid pBI221 DNA carrying the cauliflower mosaic virus promotor and bacterial β-glucuronidase coding sequence. Electrophoretic transfection is evidently effective with pieces of callus and seeds of many plants.
DOI: 10.1073/pnas.83.22.8447
发表时间: 1986-11-01
影响因子: 11.1
作者:
JEFFERSON, RA;BURGESS, SM;HIRSH, D
通讯作者: HIRSH, D