Optical determination of impulse conduction velocity during development of embryonic chick cervical vagus nerve bundles.
Optical determination of impulse conduction velocity during development of embryonic chick cervical vagus nerve bundles.
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胚胎鸡颈迷走神经束发育过程中脉冲传导速度的光学测定。
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
K. Kamino
中科院分区：
文献类型：
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作者：
T. Sakai;H. Komuro;Y. Katoh;H. Sasaki;Y. Momose;K. Kamino
1.采用光学多点同步记录电活动的方法，我们测定了5 - 21日龄鸡胚颈迷走神经束的传导速度，并研究了其发育变化。2.用电压敏感的mercury-rhodanine染料（NK 2761）对制备物进行染色，并通过用抽吸电极对迷走神经束末端施加短暂刺激来引发动作电位（脉冲）相关光学信号。使用12 x 12元件光电二极管阵列同时记录来自许多相邻区域的光信号。3.光信号从刺激部位以小的延迟传播。根据延迟和与电流施加电极的距离之间的关系，估计每个测试制备物中的传导速度：传导速度非常小，并且从5天胚胎年龄的约0.1 m s-1单调增加到孵化时的约0.4 m s-1。传导速度的增加与迷走神经束直径的发育增加密切相关。4.此外，我们还研究了电紧张电位的传播。空间常数非常小（200 - 450微米），并随着开发的进行而增加。5.还记录了具有两个不同分量的复合光学动作信号。它们似乎经常集中在8至12天大胚胎的制备物中。第二成分的传导速度比第一成分慢。我们认为，外观的第二个组成部分反映了变性的一个子集的轴突造成的“神经细胞死亡”在迷走神经的发展。
1. Employing an optical method for multiple‐site simultaneous recording of electrical activity, we have determined the conduction velocity in cervical vagus nerve bundles isolated from 5‐ to 21‐day‐old chick embryos, and investigated its developmental changes. 2. The preparations were stained with a voltage‐sensitive merocyanine‐rhodanine dye (NK2761), and action potential‐ (impulse‐) related optical signals were elicited by brief stimuli applied to the end of the vagus nerve bundle with a suction electrode. Optical signals were recorded simultaneously from many contiguous regions using a 12 x 12‐element photodiode array. 3. The optical signals spread with small delay from the site of stimulation. From the relationship between the delay and distance from the current‐applying electrode, conduction velocities were estimated in each tested preparation: the conduction velocity was very small and increased monotonically from about 0.1 m s‐1 at 5 days embryonic age to about 0.4 m s‐1 by hatching. The increase in the conduction velocity was closely related to a developmental increase in the diameter of the vagus nerve bundle. 4. In addition, we have examined the spread of electrotonic potentials. The space constant was very small (200‐450 microns) and increased as development proceeded. 5. Compound optical action signals having two distinct components were also recorded. They often appeared to be concentrated in the preparations from 8‐ to 12‐day‐old embryos. The conduction velocity of the second component was slower than that of the first. We suggest that appearance of the second component reflects degeneration of a subset of axons resulting from ‘neural cell death’ during the development of the vagus nerve.
光学记录神经末梢和精细神经元过程中的电压变化。
影响因子：
18.2
作者：
Salzberg,BM
通讯作者：
Salzberg,BM