Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation.
Involvement of Rab6a in organelle rearrangement and cytoskeletal organization during mouse oocyte maturation.
复制标题
Rab6a 参与小鼠卵母细胞成熟过程中的细胞器重排和细胞骨架组织。
DOI：
10.1038/srep23560
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发表时间：
2016-03-31
影响因子：
4.6
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Ma R;Zhang J;Liu X;Li L;Liu H;Rui R;Gu L;Wang Q
据报道，Rab GTP酶可确定囊泡的身份和运输途径。Rab6是研究最广泛的Rab蛋白之一，参与调节细胞器运输和完整性维护。然而，迄今为止，Rab6在哺乳动物卵母细胞中的功能尚未得到解决。在这里，我们报告严重的内质网紊乱后，特定的敲低Rab6a在小鼠卵母细胞。与这一发现一致，细胞内Ca2+商店相应地减少Rab6a耗尽的卵母细胞。此外，在这些卵母细胞中，我们观察到缺乏皮质颗粒自由结构域，这是成熟卵母细胞中的一种特殊细胞器，其胞吐作用是钙依赖的。另一方面，Rab6a基因敲除后，在卵母细胞减数分裂过程中检测到细胞骨架结构的显著缺陷。特别是，大多数Rab6a耗尽的卵母细胞不能形成肌动蛋白帽，纺锤体缺陷和染色体错位的频率显着升高。总之，我们的数据表明，Rab6a不仅参与调节卵母细胞的细胞器的组织，但也是一个新的调节器的减数分裂器在哺乳动物卵母细胞。
Rab GTPases have been reported to define the identity and transport routes of vesicles. Rab6 is one of the most extensively studied Rab proteins involved in regulating organelle trafficking and integrity maintenance. However, to date, the function of Rab6 in mammalian oocytes has not been addressed. Here we report severe disorganization of endoplasmic reticulum upon specific knockdown of Rab6a in mouse oocytes. In line with this finding, intracellular Ca2+ stores are accordingly reduced in Rab6a-depleted oocytes. Furthermore, in these oocytes, we observe the absence of cortical granule free domain, which is a kind of special organelle in matured oocytes and its exocytosis is calcium dependent. On the other hand, following Rab6a knockdown, the prominent defects of cytoskeletal structures are detected during oocyte meiosis. In particular, the majority of Rab6a-depleted oocytes fail to form the actin cap, and the frequency of spindle defects and chromosome misalignment is significantly elevated. In summary, our data reveal that Rab6a not only participates in modulating the organization of oocyte organelles, but also is a novel regulator of meiotic apparatus in mammalian oocytes.