Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF-κB/MAPK pathway
Exopolysaccharide from Paecilomyces lilacinus modulates macrophage activities through the TLR4/NF-κB/MAPK pathway
复制标题
淡紫拟青霉胞外多糖通过 TLR4/NF-kappa B/MAPK 途径调节巨噬细胞活性
DOI：
10.3892/mmr.2019.10746
复制
发表时间：
2019-12-01
影响因子：
3.4
通讯作者：
Tan, Guang-Hong
中科院分区：
文献类型：
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作者：
He, Chao;Lin, Hai-Yan;Tan, Guang-Hong
多种胞外多糖(EPS)是从极端环境中的各种生物中分离出来的，具有多种活性。本研究评价了从中国南部热带高盐环境中分离的淡紫色拟青霉PH0016产生的胞外多糖(PH-EPS)的免疫调节能力。以巨噬细胞RAW-264.7细胞为研究对象，探讨PH-EPS诱导巨噬细胞活化的机制。结果表明，PH-EPS能激活RAW-264.7巨噬细胞，其作用略逊于脂多糖，表现为分泌IL-1β、肿瘤坏死因子-α和一氧化氮，并显著增加吞噬功能。在PH-EPS处理的细胞中，核因子-kappaBp65显著移位到细胞核中。此外，PH-EPS处理的细胞中诱导型一氧化氮合酶(INOS)的表达和I-kappa B-α的降解均增强。PH-EPS处理的细胞p38、JNK和ERK的磷酸化水平也显著增加。此外，当丝裂原活化蛋白激酶(MAPK)通路被抑制剂Dectin-1和Toll样受体4(TLR4)抗体阻断时，PH-EPS处理的细胞中IL-1β和TNF-α的产生显著减少。结果表明，淡紫拟青霉PH-EPS具有通过TLR4/NF-kappaB/MAPKs信号通路激活RAW 264.7细胞的能力。
Multiple exopolysaccharides (EPSs) have been isolated from various organisms in extreme environments and have yielded a variety of activities. The present study evaluated the immunomodulatory capabilities of an EPS (termed PH-EPS) derived from the fungus Paecilomyces lilacinus PH0016, which was isolated from a tropical and hyperhaline environment in southern China. The macrophage RAW 264.7 cell line was used to investigate the mechanism of PH-EPS-induced macrophage activation. The results indicated that RAW 264.7 macrophages were activated by PH-EPS, in an effect slightly inferior to lipopolysaccharide (LPS), as evidenced by secretion of interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha and nitric oxide (NO), and by significantly increased phagocytosis in the cells treated with PH-EPS. Nuclear factor (NF)-kappa B p65 was significantly translocated into the nucleus in the PH-EPS-treated cells. In addition, expression of inducible NO synthase (iNOS) and I kappa B-alpha degradation were enhanced in PH-EPS-treated cells. The phosphorylation levels of p38, JNK and ERK were also significantly increased in the PH-EPS-treated cells. Furthermore, IL-1 beta and TNF-alpha production was markedly decreased in PH-EPS-treated cells when the mitogen-activated protein kinase (MAPK) pathways were blocked by the inhibitor Dectin-1 and by antibodies against Toll-like receptor 4 (TLR4). The present results indicated that PH-EPS from Paecilomyces lilacinus possessed the capability of activating RAW 264.7 cells via the TLR4/NF-kappa B/MAPKs signaling pathway.