Expression of seven main Rho family members in gastric carcinoma
Expression of seven main Rho family members in gastric carcinoma
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DOI：
10.1016/j.bbrc.2004.01.108
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发表时间：
2004-03-12
影响因子：
3.1
通讯作者：
Fan, DM
中科院分区：
文献类型：
--
作者：
Pan, YL;Bi, F;Fan, DM
Rho gtpase在癌症的发生和发展中具有重要作用，包括细胞转化、增殖、侵袭、转移和血管生成。然而，关于Rho GTPases在人类癌症标本中表达的临床意义的信息仍然很少。本研究采用半定量逆转录pcr技术，系统研究了Rho家族7个主要成员RhoA、RhoB、RhoC、Rac1、Rac2、Rac3和Cdc42在53例胃癌患者和7株胃癌细胞系中的mRNA表达水平。测定了5种胃癌细胞系中RhoA、Rac1和Cdc42的总量和活性。胃癌组织标本中RhoA和Rac1 mRNA的平均表达量显著高于癌旁非肿瘤组织标本（P < 0.01）。RhoA的高表达与胃癌TNM分期升高（p < 0.05）及多分化组织学类型呈正相关（p < 0.05）。Rac1表达升高与胃癌TNM分期升高有关（p < 0.05）。7种胃癌细胞系中RhoA和Rac1 mRNA、总蛋白表达水平及活性均高于胃粘膜上皮细胞系GES-1。这些发现提示RhoA和Rac1可能在胃癌的发生发展中起重要作用。(C) 2004爱思唯尔公司版权所有。
Rho GTPases were previously shown to have an important role in cancer development and progression, including cell transformation, proliferation, invasion, metastasis, and angiogenesis. However, there is still little information available on the clinical significance of Rho GTPases expression in human cancer specimens. In the present study, we systemically investigated the mRNA expression levels of seven main members RhoA, RhoB, RhoC, Rac1, Rac2, Rac3, and Cdc42 of Rho family using semi-quantitative reverse transcription-PCR in 53 patients with gastric carcinoma and 7 gastric cancer cell lines. The total and activities of RhoA, Rac1 and Cdc42 in 5 gastric cancer cell lines were also examined. The mean mRNA expression levels of RhoA and Rac1 in gastric cancer tissue specimens were significantly higher than those in the adjacent non-tumorous tissue specimens (P < 0.01). The higher expression of RhoA was significantly correlated with higher TNM stage (p < 0.05) as well as with pooly differentiated histological type (p < 0.05) of gastric carcinoma. The increased expression of Rac1 was related to higher TNM stages of gastric carcinoma (p < 0.05). The expression levels of mRNA, total protein and activities of RhoA and Rac1 in 7 gastric cancer cell lines were all higher than that in gastric mucosal epithelial cell line GES-1. These findings indicate that RhoA and Rac1 may play important roles in the carcinogenesis and progression of gastric carcinoma. (C) 2004 Elsevier Inc. All rights reserved.